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| Main Authors: | , , |
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| Format: | Recurso digital |
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Zenodo
2025
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| Online Access: | https://doi.org/10.5281/zenodo.17083424 |
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Table of Contents:
- <p><span>Rabies is a lethal zoonotic disease that is caused by the rabies virus (RABV). </span><span>Although vaccines have been widely used</span><span>, the dynamic characteristics of humoral immunity are still unclear.</span> H<span>ere</span>, w<span>e collected the blood in volunteers vaccinated with RABV vaccine in Essen regime</span>, and t<span>he RABV-specific i</span>mmunoglobulin <span>G (</span>IgG<span>)</span> <span>and </span><span>neutralizing antibodie</span><span>s (nAbs) were detected for immunogenicity evaluation, and B cells were determined by single-cell sequencing. </span><span>The results showed that RABV-IgG rose continuously after the fourth and fifth doses of vaccine, while nAbs reached the peak after the third. Analogously, the memory B cells presented an increasing trend, whereas </span>amplified <span>B cells mainly occurred on 14 and 28 days. The functional module related antibody productions distinctly upregulated, and some genes such as </span><em><span>JUNB</span></em> <span>and </span><em><span>BACH2</span></em><em><span>,</span></em><em> etc</em><em><span>.</span></em> <span>changed significantly among four identified B cell subsets. </span><span>Further, the omics analysis revealed memory B cells underwent rapid clonal expansion and somatic mutations </span>promoting recognition of antibody spectrum patterns <span>under vaccine stimulation.</span> <span>Overall, this study provides a novel insight for </span>understanding of immune responses <span>provoking by multi-vaccination, and benefit</span> <span>to explore the reduction of </span><span>vaccination frequency</span>.</p>