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2025
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| Online Access: | https://doi.org/10.5281/zenodo.17226880 |
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| author | TSIOURI, OLGA |
| author_facet | TSIOURI, OLGA |
| contents | <p><span lang="EN-US">This dataset contains Raw illumina RNAseq data used for genome annotation:</span></p> <p><span lang="EN-US"><strong>RNA-22</strong> and <strong>RNA-24</strong>: + <em>Lotus japonicus</em> / + <em>Fusarium solani strain K</em> samples.</span></p> <p><span lang="EN-US"><strong>RNA-25-RNA-27:</strong> - <em>Lotus japonicus</em> / + <em>Fusarium solani strain K</em> samples.</span></p> <p><span lang="EN-US">All samples where used for transcript de novo prediction using Trinity.</span></p> <p><span lang="EN-US"><strong>RNA-25</strong> was used for genome guided transcript prediction using the same software.</span></p> <p><span lang="EN-US">The resulted fasta files from the de novo and genome guided predictions were combined and imported in funannotate as transcript evidence.</span></p> <p><em><span lang="EN-US">Fusarium solani</span></em><span lang="EN-US"> strain K (FsK) is an endophytic fungus with a wide host range that protects its host plants against pathogens and environmental stresses. In this study, we performed <em>de novo</em> genome sequencing and annotation, while a phylogenomic analysis confirmed the placement of FsK within the <em>Fusarium solani</em> species complex (FSSC). A comprehensive comparative genomics analysis was conducted with species that can colonize tomato as a common host, namely the beneficial basidiomycete <em>Serendipita indica</em> and the arbuscular mycorrhizal fungus <em>Rhizophagus irregularis, </em>both model symbiotic organisms in their respective fungal divisions, as well as the closely pathogenic <em>Fusarium vanettenii</em> 77-13-4. To identify mechanisms of early-stage FsK-plant interaction and fungal adaptation, comparative analysis of secreted effectors, carbohydrate-active enzymes and secondary metabolite clusters, was performed. FsK specific genes implicated in DNA repair and iron acquisition via ferrirhodin synthesis, highlighting adaptation to stress conditions and possible mutualistic functions with plant hosts were identified. These findings provide valuable insights into the genomic determinants of fungal lifestyles within the FSSC and establish a foundation for future functional studies on fungal-plant interactions in agricultural contexts.</span></p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_17226880 |
| institution | Zenodo |
| language | |
| publishDate | 2025 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | Comparative analysis towards the identification of genome wide characteristics of a beneficial fungal endophyte-RNAseq data TSIOURI, OLGA <p><span lang="EN-US">This dataset contains Raw illumina RNAseq data used for genome annotation:</span></p> <p><span lang="EN-US"><strong>RNA-22</strong> and <strong>RNA-24</strong>: + <em>Lotus japonicus</em> / + <em>Fusarium solani strain K</em> samples.</span></p> <p><span lang="EN-US"><strong>RNA-25-RNA-27:</strong> - <em>Lotus japonicus</em> / + <em>Fusarium solani strain K</em> samples.</span></p> <p><span lang="EN-US">All samples where used for transcript de novo prediction using Trinity.</span></p> <p><span lang="EN-US"><strong>RNA-25</strong> was used for genome guided transcript prediction using the same software.</span></p> <p><span lang="EN-US">The resulted fasta files from the de novo and genome guided predictions were combined and imported in funannotate as transcript evidence.</span></p> <p><em><span lang="EN-US">Fusarium solani</span></em><span lang="EN-US"> strain K (FsK) is an endophytic fungus with a wide host range that protects its host plants against pathogens and environmental stresses. In this study, we performed <em>de novo</em> genome sequencing and annotation, while a phylogenomic analysis confirmed the placement of FsK within the <em>Fusarium solani</em> species complex (FSSC). A comprehensive comparative genomics analysis was conducted with species that can colonize tomato as a common host, namely the beneficial basidiomycete <em>Serendipita indica</em> and the arbuscular mycorrhizal fungus <em>Rhizophagus irregularis, </em>both model symbiotic organisms in their respective fungal divisions, as well as the closely pathogenic <em>Fusarium vanettenii</em> 77-13-4. To identify mechanisms of early-stage FsK-plant interaction and fungal adaptation, comparative analysis of secreted effectors, carbohydrate-active enzymes and secondary metabolite clusters, was performed. FsK specific genes implicated in DNA repair and iron acquisition via ferrirhodin synthesis, highlighting adaptation to stress conditions and possible mutualistic functions with plant hosts were identified. These findings provide valuable insights into the genomic determinants of fungal lifestyles within the FSSC and establish a foundation for future functional studies on fungal-plant interactions in agricultural contexts.</span></p> |
| title | Comparative analysis towards the identification of genome wide characteristics of a beneficial fungal endophyte-RNAseq data |
| url | https://doi.org/10.5281/zenodo.17226880 |