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| Formato: | Recurso digital |
| Lenguaje: | inglés |
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2025
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| Acceso en línea: | https://doi.org/10.5281/zenodo.17228047 |
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| _version_ | 1866902082113503232 |
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| author | Butnor, John |
| author_facet | Butnor, John |
| contents | <p>These datasets and associated program files support a manuscript to be submitted to HardwareX journal titled: "A simple prototype for assessing plant cold hardiness with differential thermal analysis" by John R Butnor, USDA Forest Service, Northern Research Station, Burlington, VT, USA.</p> <p>They are structured into 4 zip files:</p> <p><strong>CR23X program files.zip</strong> contains program files associated with a Campbell Scientific logger CR23X</p> <p><strong>CR1000X program files.zip</strong> contains program files associated with a Campbell Scientific logger CR1000X</p> <p><strong>interpretation.zip</strong> contains files related to Differential Thermal Analysis interpretation. The R program used to generate self contained html fils for Differential Thermal Analysis (DTA) is named "DTA interpretation.R" and the associated data set is named "sample_data_09022025.csv". The generated files are:</p> <p>"sample_data_09022025.pdf"</p> <p>"temp_cleaned.csv"</p> <p>"TEM_1_MAX_example_column_3.html"</p> <p>"TEM_2_MAX_example_column_4.html"</p> <p>"TEM_3_MAX_example_column_5.html"</p> <p>"TEM_4_MAX_example_column_6.html"</p> <p>"TEM_5_MAX_example_column_7.html"</p> <p><strong>red_spruce_example.zip </strong>contains R program, data and related outputs associated with a biological case example of DTA analysis with red spruce. The files are similar to those found in interpretation.zip above, but specific to the experiment described below:</p> <p>Dormant buds of red spruce (<em>Picea rubens</em> Sarg.) were used to demonstrate the ability of the DTA prototype to detect exotherms produced by plant tissue at specific temperatures. On May 19, 2024, dormant red spruce buds were collected from 2 elevations (549 m and 1,067 m) on Mount Ellen in northern Vermont and one elevation (69 m) at the University of Vermont (UVM) Horticultural Education and Research Center in South Burlington Vermont. Foliage samples from 549 m and 1,067 m were also collected to utilize the remaining TEM wells. The samples were stored in a refrigerator until running the system the next day. The rationale of sampling trees at different elevations was to demonstrate that trees were becoming less cold tolerant in response to warming springtime ambient temperatures at each elevation. Accordingly, samples collected from a lower, warmer elevation would be less cold tolerant and therefore produce exotherms at warmer temperatures upon incremental freezing. Likewise, samples collected from a higher, colder elevation would be expected to more cold tolerant and exhibit exotherms at comparatively lower temperatures with controlled freezing.</p> <p>The prototype was controlled by a Campbell Scientific Inc. (Logan, UT, USA) model CR23X datalogger with programs created with the ShortCut utility: *.SCW which summarizes all options selected in the ShortCut GUI, *.DEF defines the physical wiring locations on the logger and names the data output labels, and *.DLD which is the compiled machine language that is sent to and used by the datalogger. The prototype control program measures all TEMs every second, and logs maximum and mean millivolt values every 10 seconds.</p> <p>A programable ultra-low temperature chamber (MC-812, ESPEC North America, Inc., 4141 Central Parkway, Hudsonville, MI 49426 USA) was pre-cooled to 4°C. The DTA prototype was placed in the freezer with red spruce tissue place atop TEMs and the control program was initiated. Once the champer temperature was steady, it was programmed to soak (hold constant) at 4°C for 12 hours and then ramp (controlled transition) temperatures down at a rate of -4°C per hour until -40°C was reached. </p> <p>The file headers in the attached dataset are: TIMESTAMP RECORD T65P_MAX T65_MAX T127_MAX T109P_MAX T109_MAX Temp_1_MAX Temp_2_MAX T65P_AVG T65_AVG T127_AVG T109P_AVG T109_AVG Temp_1_AVG Temp_2_AVG</p> <p><br> The red spruce tissue sample placed on TEMs as follows:</p> <p>T65P buds collected at 1067 m</p> <p>T65 buds collected at 549 m</p> <p>T127 foliage collected at 69 m</p> <p>T109P buds collected at 69 m</p> <p>T109 foliage collected at 1067 m</p> <p> </p> <p> </p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_17228047 |
| institution | Zenodo |
| language | eng |
| publishDate | 2025 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | A simple prototype for assessing plant cold hardiness with differential thermal analysis Butnor, John differential thermal analysis DTA red spruce Picea rubens freezing injury <p>These datasets and associated program files support a manuscript to be submitted to HardwareX journal titled: "A simple prototype for assessing plant cold hardiness with differential thermal analysis" by John R Butnor, USDA Forest Service, Northern Research Station, Burlington, VT, USA.</p> <p>They are structured into 4 zip files:</p> <p><strong>CR23X program files.zip</strong> contains program files associated with a Campbell Scientific logger CR23X</p> <p><strong>CR1000X program files.zip</strong> contains program files associated with a Campbell Scientific logger CR1000X</p> <p><strong>interpretation.zip</strong> contains files related to Differential Thermal Analysis interpretation. The R program used to generate self contained html fils for Differential Thermal Analysis (DTA) is named "DTA interpretation.R" and the associated data set is named "sample_data_09022025.csv". The generated files are:</p> <p>"sample_data_09022025.pdf"</p> <p>"temp_cleaned.csv"</p> <p>"TEM_1_MAX_example_column_3.html"</p> <p>"TEM_2_MAX_example_column_4.html"</p> <p>"TEM_3_MAX_example_column_5.html"</p> <p>"TEM_4_MAX_example_column_6.html"</p> <p>"TEM_5_MAX_example_column_7.html"</p> <p><strong>red_spruce_example.zip </strong>contains R program, data and related outputs associated with a biological case example of DTA analysis with red spruce. The files are similar to those found in interpretation.zip above, but specific to the experiment described below:</p> <p>Dormant buds of red spruce (<em>Picea rubens</em> Sarg.) were used to demonstrate the ability of the DTA prototype to detect exotherms produced by plant tissue at specific temperatures. On May 19, 2024, dormant red spruce buds were collected from 2 elevations (549 m and 1,067 m) on Mount Ellen in northern Vermont and one elevation (69 m) at the University of Vermont (UVM) Horticultural Education and Research Center in South Burlington Vermont. Foliage samples from 549 m and 1,067 m were also collected to utilize the remaining TEM wells. The samples were stored in a refrigerator until running the system the next day. The rationale of sampling trees at different elevations was to demonstrate that trees were becoming less cold tolerant in response to warming springtime ambient temperatures at each elevation. Accordingly, samples collected from a lower, warmer elevation would be less cold tolerant and therefore produce exotherms at warmer temperatures upon incremental freezing. Likewise, samples collected from a higher, colder elevation would be expected to more cold tolerant and exhibit exotherms at comparatively lower temperatures with controlled freezing.</p> <p>The prototype was controlled by a Campbell Scientific Inc. (Logan, UT, USA) model CR23X datalogger with programs created with the ShortCut utility: *.SCW which summarizes all options selected in the ShortCut GUI, *.DEF defines the physical wiring locations on the logger and names the data output labels, and *.DLD which is the compiled machine language that is sent to and used by the datalogger. The prototype control program measures all TEMs every second, and logs maximum and mean millivolt values every 10 seconds.</p> <p>A programable ultra-low temperature chamber (MC-812, ESPEC North America, Inc., 4141 Central Parkway, Hudsonville, MI 49426 USA) was pre-cooled to 4°C. The DTA prototype was placed in the freezer with red spruce tissue place atop TEMs and the control program was initiated. Once the champer temperature was steady, it was programmed to soak (hold constant) at 4°C for 12 hours and then ramp (controlled transition) temperatures down at a rate of -4°C per hour until -40°C was reached. </p> <p>The file headers in the attached dataset are: TIMESTAMP RECORD T65P_MAX T65_MAX T127_MAX T109P_MAX T109_MAX Temp_1_MAX Temp_2_MAX T65P_AVG T65_AVG T127_AVG T109P_AVG T109_AVG Temp_1_AVG Temp_2_AVG</p> <p><br> The red spruce tissue sample placed on TEMs as follows:</p> <p>T65P buds collected at 1067 m</p> <p>T65 buds collected at 549 m</p> <p>T127 foliage collected at 69 m</p> <p>T109P buds collected at 69 m</p> <p>T109 foliage collected at 1067 m</p> <p> </p> <p> </p> |
| title | A simple prototype for assessing plant cold hardiness with differential thermal analysis |
| topic | differential thermal analysis DTA red spruce Picea rubens freezing injury |
| url | https://doi.org/10.5281/zenodo.17228047 |