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| Main Authors: | , , , , , |
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| Format: | Recurso digital |
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Zenodo
2025
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| Online Access: | https://doi.org/10.5281/zenodo.17296197 |
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Table of Contents:
- <p>This repository provides all supplementary movies associated with the publication “<em>Capturing the Beat: Quantification of Flagellar Kinematics and 3D Beat Patterns in Pseudobodo sp. Using Digital Holographic Microscopy</em>.”</p> <p>Description of each data file:</p> <p><strong><span lang="EN-US">Movie S1. </span></strong><em><span lang="EN-US">Pseudobodo sp.</span></em><span lang="EN-US"> swimming with an almost entirely planar flagellar beat, propagating a smooth undulatory wave within a single plane. The left panel shows the reconstructed single-plane phase <em>z</em>-stack sequence with tracked flagellar points (light green) and the attachment point (red). The region highlighted by the orange rectangle denotes the region enlarged in the middle panel. The right panel presents the 3D reconstruction of the beating flagellum, illustrating the planarity of the waveform.</span></p> <p><strong><span lang="EN-US">Movie S2. </span></strong><em><span lang="EN-US">Pseudobodo sp.</span></em><span lang="EN-US"> swimming with a near-planar flagellar beat, with the beating plane oriented almost perpendicular to the <em>XY</em> plane. The top left panel shows the <em>XY</em> projection, the top right panel displays the 3D reconstruction, and the bottom left and bottom right panels present the <em>XZ</em> and <em>YZ</em> projections, respectively. In all panels, tracked flagellar points are shown in light green and the attachment point in red.</span></p> <p><strong><span lang="EN-US">Movie S3. </span></strong><span lang="EN-US">During feeding, <em>Pseudobodo sp.</em> displays a non-planar, tortuous flagellar beat that sweeps through a large 3D space, likely enhancing prey encounter rates. The left panel shows the reconstructed single-plane phase <em>z</em>-stack sequence with tracked flagellar points (blue) and the attachment point (red). The orange rectangle denotes the region enlarged in the middle panel. The right panel shows the 3D reconstruction of the beating flagellum, illustrating its expanded spatial envelope.</span></p> <p><strong><span lang="EN-US">Movie S4. </span></strong><span lang="EN-US">During feeding, <em>Pseudobodo sp.</em> adopts a fully 3D flagellar waveform, reflecting its role in generating flows that draw prey toward the cell body. The top left panel shows the <em>XY</em> projection, the top right panel displays the 3D reconstruction, and the bottom left and bottom right panels present the <em>YZ</em> and <em>XZ</em> projections, respectively. In the <em>XZ</em> projection, the flagellum forms a distinct lasso-shaped loop with a diameter of ~6 µm, a feature obscured in the <em>XY</em> projection. In all panels, tracked flagellar points are shown in blue and the attachment point in red.</span></p> <p><strong><span lang="EN-US">Movie S5. </span></strong><em><span lang="EN-US">Pseudobodo sp.</span></em><span lang="EN-US"> actively modulates the 3D kinematics of its anterior flagellum, likely as an adaptive strategy to the propulsion–foraging–predation-risk trade-off. The left panel shows the swimming mode, where the flagellum executes a tightly constrained, nearly planar undulatory beat within a narrow sheet of water (flagellar points in light green, attachment point in red). The right panel shows the feeding mode, characterized by a wide-sweeping, non-planar motion that generates strong feeding currents and enhances prey encounter rates (flagellar points in blue, attachment point in red).</span></p>