Association between breast cancer susceptibility and variation in the catalase gene in women from Babylon, Iraq

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Autor principal: Salah Hashim Shaheed
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Publicado: Zenodo 2025
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author Salah Hashim Shaheed
author_facet Salah Hashim Shaheed
contents <p>Catalase0represents a critical enzyme in the organism's defense system against oxidative stress, a physiolog1qaical state<br>implicated in the pathogenesis of various cancers. As a primary antioxidant defense enzyme, catalase plays a vital role in mitigating<br>oxidative damage. Substantial evidence from numerous studies indicates that genetic polymorphisms in the CAT gene are significant<br>in cancer etiology. This investigation aimed to elucidate the influence of the CAT gene polymorphism0(rs7943316)0on breast cancer<br>susceptibility. Genomic DNA was isolated from blood samples of breast cancer patients and a control cohort. The specified0singlenucleotide0polymorphism (SNP) was analyzed using polymerase.chain reaction (PCR) coupled with restriction<br>fragment0length.polymorphism (RFLP)0analysis. Genotype distribution in the control group was as follows: the heterozygous A/T<br>genotype was most prevalent0(55 %), followed0by the homozygous T/T genotype (36.7%), while the homozygous A/A genotype was<br>not0observed (8.3 %). In the breast cancer (BC) patient group, the A/T0genotype was also the most frequent (51.7%), followed by T/T<br>(31.7%) and A/A (16.6%).Analysis revealed that individuals carrying the A/T and T/T genotypes exhibited an elevated odds ratio for<br>breast cancer development (OR = 2.129, P = 0.209 and OR = 2.315, P = 0.183, respectively). However, these associations were not<br>statistically significant. Furthermore, comparative allele frequency analysis showed no significant difference in the distribution of<br>the0T.allele of the CAT gene (.rs7943316)0between the breast cancer0patients and the control group (P = 0.290). In conclusion, the<br>findings of this study indicate that the development of breast cancer is not associated with polymorphisms of the CAT gene at the<br>rs7943316 locus.</p>
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publishDate 2025
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spellingShingle Association between breast cancer susceptibility and variation in the catalase gene in women from Babylon, Iraq
Salah Hashim Shaheed
rs7943316; SNP promoter; Breast Cancer Susceptibility; Oxidative Stress.
<p>Catalase0represents a critical enzyme in the organism's defense system against oxidative stress, a physiolog1qaical state<br>implicated in the pathogenesis of various cancers. As a primary antioxidant defense enzyme, catalase plays a vital role in mitigating<br>oxidative damage. Substantial evidence from numerous studies indicates that genetic polymorphisms in the CAT gene are significant<br>in cancer etiology. This investigation aimed to elucidate the influence of the CAT gene polymorphism0(rs7943316)0on breast cancer<br>susceptibility. Genomic DNA was isolated from blood samples of breast cancer patients and a control cohort. The specified0singlenucleotide0polymorphism (SNP) was analyzed using polymerase.chain reaction (PCR) coupled with restriction<br>fragment0length.polymorphism (RFLP)0analysis. Genotype distribution in the control group was as follows: the heterozygous A/T<br>genotype was most prevalent0(55 %), followed0by the homozygous T/T genotype (36.7%), while the homozygous A/A genotype was<br>not0observed (8.3 %). In the breast cancer (BC) patient group, the A/T0genotype was also the most frequent (51.7%), followed by T/T<br>(31.7%) and A/A (16.6%).Analysis revealed that individuals carrying the A/T and T/T genotypes exhibited an elevated odds ratio for<br>breast cancer development (OR = 2.129, P = 0.209 and OR = 2.315, P = 0.183, respectively). However, these associations were not<br>statistically significant. Furthermore, comparative allele frequency analysis showed no significant difference in the distribution of<br>the0T.allele of the CAT gene (.rs7943316)0between the breast cancer0patients and the control group (P = 0.290). In conclusion, the<br>findings of this study indicate that the development of breast cancer is not associated with polymorphisms of the CAT gene at the<br>rs7943316 locus.</p>
title Association between breast cancer susceptibility and variation in the catalase gene in women from Babylon, Iraq
topic rs7943316; SNP promoter; Breast Cancer Susceptibility; Oxidative Stress.
url https://doi.org/10.5281/zenodo.17336794