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Zenodo
2025
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| Online Access: | https://doi.org/10.5281/zenodo.17399249 |
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| _version_ | 1866902199191207936 |
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| author | Zhang, Yingying Watson, Brianna Rattan, Ajitanuj Lee, Tyrone Chawla, Smriti Geistlinger, Ludwig Guan, Yilin Lord, Finley Ma, Minghe Miwa, Takashi Golla, Madhu Caldarone, Barbara Song, Wen-chao Moffitt, Jeffrey Carroll, Michael |
| author_facet | Zhang, Yingying Watson, Brianna Rattan, Ajitanuj Lee, Tyrone Chawla, Smriti Geistlinger, Ludwig Guan, Yilin Lord, Finley Ma, Minghe Miwa, Takashi Golla, Madhu Caldarone, Barbara Song, Wen-chao Moffitt, Jeffrey Carroll, Michael |
| contents | <p>Recent studies have found non-immunological roles of the classical complement pathway (CP) in brain development and its involvement in neuropsychiatric and neurodegenerative diseases. However, multiple complement activation pathways exist beyond the CP, but their expression and function remain poorly understood in the brain. Using MERFISH, we constructed a comprehensive spatial transcriptomic atlas of the complement system in mouse brains from the late embryonic stage to adulthood. Here, we show that most complement genes are expressed locally with a remarkable degree of cellular, spatial, and temporal heterogeneity and that complement regulatory mechanisms are distinct from the periphery. Beyond confirming the known expression of the CP, our measurements reveal endogenous expression of the alternative pathway (AP), notably the AP activator Masp3 in immature brains. Masp3 deficiency causes working spatial memory defects and altered molecular structure of the brain, indicating a role of Masp3 in brain maturation, potentially via modulation of AP activity.</p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_17399249 |
| institution | Zenodo |
| language | |
| publishDate | 2025 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | A spatial atlas of the complement system uncovers unique expression patterns in postnatal brain development in mice Zhang, Yingying Watson, Brianna Rattan, Ajitanuj Lee, Tyrone Chawla, Smriti Geistlinger, Ludwig Guan, Yilin Lord, Finley Ma, Minghe Miwa, Takashi Golla, Madhu Caldarone, Barbara Song, Wen-chao Moffitt, Jeffrey Carroll, Michael Spatial transcriptomics MERFISH Brain development complement Alternative pathway Masp3 <p>Recent studies have found non-immunological roles of the classical complement pathway (CP) in brain development and its involvement in neuropsychiatric and neurodegenerative diseases. However, multiple complement activation pathways exist beyond the CP, but their expression and function remain poorly understood in the brain. Using MERFISH, we constructed a comprehensive spatial transcriptomic atlas of the complement system in mouse brains from the late embryonic stage to adulthood. Here, we show that most complement genes are expressed locally with a remarkable degree of cellular, spatial, and temporal heterogeneity and that complement regulatory mechanisms are distinct from the periphery. Beyond confirming the known expression of the CP, our measurements reveal endogenous expression of the alternative pathway (AP), notably the AP activator Masp3 in immature brains. Masp3 deficiency causes working spatial memory defects and altered molecular structure of the brain, indicating a role of Masp3 in brain maturation, potentially via modulation of AP activity.</p> |
| title | A spatial atlas of the complement system uncovers unique expression patterns in postnatal brain development in mice |
| topic | Spatial transcriptomics MERFISH Brain development complement Alternative pathway Masp3 |
| url | https://doi.org/10.5281/zenodo.17399249 |