Supplementary data: Overriding bioprocess perturbations with a cell-machine interface for reliable microbial stress-response control
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2025
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| author | Delvenne, Matheo Martínez Alvarez, Juan Andrés Haringa, Cees Noorman, Henk Minden, Steven Takors, Ralf Delvigne, Frank |
| author_facet | Delvenne, Matheo Martínez Alvarez, Juan Andrés Haringa, Cees Noorman, Henk Minden, Steven Takors, Ralf Delvigne, Frank |
| contents | <p lang="en-US">Supplementary data for the paper:</p> <p><strong>Overriding bioprocess perturbations with a cell-machine interface for reliable microbial stress-response control</strong></p> <p lang="en-US"><em>Abstract</em></p> <p lang="en-US">Controlling cell population dynamics and phenotypic diversification is a key objective in systems and synthetic biology, particularly for ensuring uniform responses from engineered gene circuits. While cell-machine interfaces have been employed to modulate host-gene circuit interactions, environmental perturbations typical of industrial bioreactor conditions remain underexplored. In this study, we investigate the impact of such perturbations on the general stress response in <em>Escherichia coli</em> and <em>Saccharomyces cerevisiae</em>. Using scale-down bioreactor experiments, we evaluate the performance of the Segregostat, a real-time control system that leverages automated flow cytometry to induce dynamic nutrient shifts. The Segregostat achieves robust stress response control, even under severe perturbations such as extended residence times in a two-compartment reactor. We hypothesize that this robustness arises from the system’s ability to amplify host-compatible fluctuations beyond bioreactor-induced perturbations. Our findings highlight the importance of integrating environmental factors into control strategies for reliable gene circuit behavior in industrial bioprocessing environments.</p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_17453611 |
| institution | Zenodo |
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| publishDate | 2025 |
| publisher | Zenodo |
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| spellingShingle | Supplementary data: Overriding bioprocess perturbations with a cell-machine interface for reliable microbial stress-response control Delvenne, Matheo Martínez Alvarez, Juan Andrés Haringa, Cees Noorman, Henk Minden, Steven Takors, Ralf Delvigne, Frank microbial stress Saccharomyces cerevisiae Escherichia coli bet-hedging Segregostat automated flow cytometry Single-Cell Analysis cell lifeline large-scale bioreactor scale-down <p lang="en-US">Supplementary data for the paper:</p> <p><strong>Overriding bioprocess perturbations with a cell-machine interface for reliable microbial stress-response control</strong></p> <p lang="en-US"><em>Abstract</em></p> <p lang="en-US">Controlling cell population dynamics and phenotypic diversification is a key objective in systems and synthetic biology, particularly for ensuring uniform responses from engineered gene circuits. While cell-machine interfaces have been employed to modulate host-gene circuit interactions, environmental perturbations typical of industrial bioreactor conditions remain underexplored. In this study, we investigate the impact of such perturbations on the general stress response in <em>Escherichia coli</em> and <em>Saccharomyces cerevisiae</em>. Using scale-down bioreactor experiments, we evaluate the performance of the Segregostat, a real-time control system that leverages automated flow cytometry to induce dynamic nutrient shifts. The Segregostat achieves robust stress response control, even under severe perturbations such as extended residence times in a two-compartment reactor. We hypothesize that this robustness arises from the system’s ability to amplify host-compatible fluctuations beyond bioreactor-induced perturbations. Our findings highlight the importance of integrating environmental factors into control strategies for reliable gene circuit behavior in industrial bioprocessing environments.</p> |
| title | Supplementary data: Overriding bioprocess perturbations with a cell-machine interface for reliable microbial stress-response control |
| topic | microbial stress Saccharomyces cerevisiae Escherichia coli bet-hedging Segregostat automated flow cytometry Single-Cell Analysis cell lifeline large-scale bioreactor scale-down |
| url | https://doi.org/10.5281/zenodo.17453611 |