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2025
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| Online Access: | https://doi.org/10.5281/zenodo.17470467 |
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| author | Dr. Jyoti Chaubey |
| author_facet | Dr. Jyoti Chaubey |
| contents | <p><strong>Background and aim</strong>: Anemia is the most common hematologic abnormality identified in infants and children. The present study was conducted to evaluate the hemoglobin values in pediatric age group by Sahli’s acid hematin method, cyanmethemoglobin method and automated hematology analyser and to compare and contrast sahlis acid hematin method and automated analyser with respect to cyanmethemoglobin method for hemoglobin estimation and to evaluate the diagnostic accuracy of both.</p> <p><strong>Material and Methods</strong>: This is a cross-sectional study. Blood sample was collected in two EDTA vials from 100 outdoor patients in the age group 1 year to 12 years attending CCL Pathology. The hemoglobin values of the collected blood samples was analysed by Sahlis Acid Hematin, cyanmethemoglobin method in the Department of Physiology and Automated Hematology Analyser (Elite 580, ERBA manheim) in the Department of Pathology. The methods were compared and analysed for diagnostic accuracy through chi-square tests and ANOVA. A value of <0.05 was regarded as statistically significant.</p> <p><strong>Results</strong>: In our study, all three methods exhibit a similar level of repeatability, with standard deviations around 1.94–1.95. This indicates that all methods are consistent in their measurements with minimal variability. Sahli's Method has a narrower prediction range (3.55–11.32), which might suggest lower reliability for measurements across the full spectrum of possible values. Cyanmethemoglobin and Automated hemoglobin show wider and more similar prediction ranges, indicating better capability to accommodate a broader range of hemoglobin values.</p> <p><strong>Conclusion</strong>: In our study, all three methods exhibit a similar level of repeatability which indicates that all methods are consistent in their measurements with minimal variability. The Automated hemoglobin and Cyanmethemoglobin methods are likely more suitable for applications requiring higher reliability and coverage of a wide range of hemoglobin values. The Sahli's Method, while consistent, might be less reliable for extreme or high values due to its narrower range.</p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_17470467 |
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| publishDate | 2025 |
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| spellingShingle | Evaluation of Hemoglobin by Using Sahli's Method and Automated Analyser, A Comparative Study in Paediatric Age Group in A Tertiary Care Hospital of Assam Dr. Jyoti Chaubey <p><strong>Background and aim</strong>: Anemia is the most common hematologic abnormality identified in infants and children. The present study was conducted to evaluate the hemoglobin values in pediatric age group by Sahli’s acid hematin method, cyanmethemoglobin method and automated hematology analyser and to compare and contrast sahlis acid hematin method and automated analyser with respect to cyanmethemoglobin method for hemoglobin estimation and to evaluate the diagnostic accuracy of both.</p> <p><strong>Material and Methods</strong>: This is a cross-sectional study. Blood sample was collected in two EDTA vials from 100 outdoor patients in the age group 1 year to 12 years attending CCL Pathology. The hemoglobin values of the collected blood samples was analysed by Sahlis Acid Hematin, cyanmethemoglobin method in the Department of Physiology and Automated Hematology Analyser (Elite 580, ERBA manheim) in the Department of Pathology. The methods were compared and analysed for diagnostic accuracy through chi-square tests and ANOVA. A value of <0.05 was regarded as statistically significant.</p> <p><strong>Results</strong>: In our study, all three methods exhibit a similar level of repeatability, with standard deviations around 1.94–1.95. This indicates that all methods are consistent in their measurements with minimal variability. Sahli's Method has a narrower prediction range (3.55–11.32), which might suggest lower reliability for measurements across the full spectrum of possible values. Cyanmethemoglobin and Automated hemoglobin show wider and more similar prediction ranges, indicating better capability to accommodate a broader range of hemoglobin values.</p> <p><strong>Conclusion</strong>: In our study, all three methods exhibit a similar level of repeatability which indicates that all methods are consistent in their measurements with minimal variability. The Automated hemoglobin and Cyanmethemoglobin methods are likely more suitable for applications requiring higher reliability and coverage of a wide range of hemoglobin values. The Sahli's Method, while consistent, might be less reliable for extreme or high values due to its narrower range.</p> |
| title | Evaluation of Hemoglobin by Using Sahli's Method and Automated Analyser, A Comparative Study in Paediatric Age Group in A Tertiary Care Hospital of Assam |
| url | https://doi.org/10.5281/zenodo.17470467 |