ORGAN-SPECIFIC QUANTITATIVE PROFILING OF PRIMARY METABOLITES IN FICUS BENGHALENSIS L.

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Main Author: Dr. V. B. Kadam*1, Dr. S. M. Khan2 and V. B. Thombare3
Format: Recurso digital
Language:English
Published: Zenodo 2025
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_version_ 1866901820345942016
author Dr. V. B. Kadam*1, Dr. S. M. Khan2 and V. B. Thombare3
author_facet Dr. V. B. Kadam*1, Dr. S. M. Khan2 and V. B. Thombare3
contents <p>Primary metabolites such as proteins, carbohydrates, lipids, and free amino acids are essential for plant growth, metabolism, and nutritive/medicinal use. This study quantifies and compares primary metabolite content in four plant parts (leaves, stem bark, roots, and fruits) of Ficus benghalensis L. Mature, healthy trees were sampled, plant materials air-dried and powdered, and analyzed using standard biochemical methods (Lowry/Bradford for protein, An throne for total carbohydrate, Folch/Bligh & Dyer for total lipids, ninhydrin-based assay for free amino acids). Five biological replicates were analyzed per plant part. Significant organ-specific differences were observed (one-way ANOVA, p < 0.05). Leaves contained the highest protein (13.1 ± 0.6% DW) and free amino acids (19.2 ± 1.1 mg g⁻¹ DW). Fruits were richest in carbohydrates (57.2 ± 2.3% DW). Stem bark showed intermediate values, while roots had a high carbohydrate-to-protein ratio. Lipids were relatively low across all organs (2.0–4.5% DW). These differences reflect organ-specific metabolic allocation and have implications for nutritional use, medicinal applications, and further biochemical studies of F. benghalensis.</p>
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publishDate 2025
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spellingShingle ORGAN-SPECIFIC QUANTITATIVE PROFILING OF PRIMARY METABOLITES IN FICUS BENGHALENSIS L.
Dr. V. B. Kadam*1, Dr. S. M. Khan2 and V. B. Thombare3
Ficus benghalensis, primary metabolites, protein, carbohydrate, lipid, free amino acids, comparative biochemistry.
<p>Primary metabolites such as proteins, carbohydrates, lipids, and free amino acids are essential for plant growth, metabolism, and nutritive/medicinal use. This study quantifies and compares primary metabolite content in four plant parts (leaves, stem bark, roots, and fruits) of Ficus benghalensis L. Mature, healthy trees were sampled, plant materials air-dried and powdered, and analyzed using standard biochemical methods (Lowry/Bradford for protein, An throne for total carbohydrate, Folch/Bligh & Dyer for total lipids, ninhydrin-based assay for free amino acids). Five biological replicates were analyzed per plant part. Significant organ-specific differences were observed (one-way ANOVA, p < 0.05). Leaves contained the highest protein (13.1 ± 0.6% DW) and free amino acids (19.2 ± 1.1 mg g⁻¹ DW). Fruits were richest in carbohydrates (57.2 ± 2.3% DW). Stem bark showed intermediate values, while roots had a high carbohydrate-to-protein ratio. Lipids were relatively low across all organs (2.0–4.5% DW). These differences reflect organ-specific metabolic allocation and have implications for nutritional use, medicinal applications, and further biochemical studies of F. benghalensis.</p>
title ORGAN-SPECIFIC QUANTITATIVE PROFILING OF PRIMARY METABOLITES IN FICUS BENGHALENSIS L.
topic Ficus benghalensis, primary metabolites, protein, carbohydrate, lipid, free amino acids, comparative biochemistry.
url https://doi.org/10.5281/zenodo.17494638