In Vitro Evaluation of Ethyl Acetate Extract of Tridax Procumbens On Calcium Oxalate Crystal Dissolution: A Potential Anti‑Urolithiatic Agent
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2025
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| _version_ | 1866901241644187648 |
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| author | Atharva Patil*, Pratik Shivtare, Chetan Telangre |
| author_facet | Atharva Patil*, Pratik Shivtare, Chetan Telangre |
| contents | <p><span>Background: Urolithiasis is a common urological disorder, with calcium oxalate stones being the predominant type. Traditional medicinal plants remain a valuable source of therapeutic leads against stone formation. Objective: This in vitro study evaluated the antiurolithiatic potential of an ethyl acetate extract (EAE) of Tridax procumbens using standardized nucleation and aggregation assays, comparing activity to a marketed herbal polyherbal control, Cystone® (250 µg/mL). Methods: EAE was prepared from aerial parts of T. procumbens and tested at 100–500 µg/mL against chemically synthesized calcium oxalate monohydrate (COM) crystals. Nucleation (turbidity at 620 nm) and aggregation (optical density change at 620 nm) assays were performed in triplicate. Results: EAE exhibited concentration‑dependent inhibition of nucleation and aggregation. At 500 µg/mL, nucleation inhibition reached 78.4 ± 2.6% and aggregation inhibition 71.2 ± 3.1%, compared to Cystone® which showed 64.0 ± 2.8% and 59.5 ± 3.0% respectively. Mechanistic tests suggested Ca2+ chelation and surface-active properties as contributing factors. Conclusion: Ethyl acetate extract of T. procumbens demonstrates promising in vitro antiurolithiatic activity, surpassing Cystone® in these assays. Further bioactive‑guided isolation and in vivo validation are warranted.</span></p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_17512613 |
| institution | Zenodo |
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| publishDate | 2025 |
| publisher | Zenodo |
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| spellingShingle | In Vitro Evaluation of Ethyl Acetate Extract of Tridax Procumbens On Calcium Oxalate Crystal Dissolution: A Potential Anti‑Urolithiatic Agent Atharva Patil*, Pratik Shivtare, Chetan Telangre Tridax procumbens; calcium oxalate; urolithiasis; ethyl acetate extract; nucleation; aggregation; Cystone <p><span>Background: Urolithiasis is a common urological disorder, with calcium oxalate stones being the predominant type. Traditional medicinal plants remain a valuable source of therapeutic leads against stone formation. Objective: This in vitro study evaluated the antiurolithiatic potential of an ethyl acetate extract (EAE) of Tridax procumbens using standardized nucleation and aggregation assays, comparing activity to a marketed herbal polyherbal control, Cystone® (250 µg/mL). Methods: EAE was prepared from aerial parts of T. procumbens and tested at 100–500 µg/mL against chemically synthesized calcium oxalate monohydrate (COM) crystals. Nucleation (turbidity at 620 nm) and aggregation (optical density change at 620 nm) assays were performed in triplicate. Results: EAE exhibited concentration‑dependent inhibition of nucleation and aggregation. At 500 µg/mL, nucleation inhibition reached 78.4 ± 2.6% and aggregation inhibition 71.2 ± 3.1%, compared to Cystone® which showed 64.0 ± 2.8% and 59.5 ± 3.0% respectively. Mechanistic tests suggested Ca2+ chelation and surface-active properties as contributing factors. Conclusion: Ethyl acetate extract of T. procumbens demonstrates promising in vitro antiurolithiatic activity, surpassing Cystone® in these assays. Further bioactive‑guided isolation and in vivo validation are warranted.</span></p> |
| title | In Vitro Evaluation of Ethyl Acetate Extract of Tridax Procumbens On Calcium Oxalate Crystal Dissolution: A Potential Anti‑Urolithiatic Agent |
| topic | Tridax procumbens; calcium oxalate; urolithiasis; ethyl acetate extract; nucleation; aggregation; Cystone |
| url | https://doi.org/10.5281/zenodo.17512613 |