Analytical Method Development and Validation by HPTLC
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2025
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| author | Reshma Lende*, Monali Khatake, Akshada Gadakh |
| author_facet | Reshma Lende*, Monali Khatake, Akshada Gadakh |
| contents | <p>High Performance Thin Layer Chromatography (HPTLC) is an advanced form of Thin Layer Chromatography (TLC), used for both qualitative and quantitative analysis of compounds. It offers better separation, sensitivity, and automation compared to traditional TLC. HPTLC works on the principle of differential migration, where substances are separated based on their interaction with the stationary phase (usually silica gel) and a chosen mobile phase (solvent system). Key components of HPTLC instrumentation include an auto-sampler, developing chamber, digital imaging system, and a TLC scanner, all of which improve accuracy and efficiency. The method development process includes steps such as sample preparation, selection of stationary and mobile phases, plate pre-washing, sample application, chromatogram development, and detection. Detection is done using UV light or chemical derivatization, followed by quantification using a densitometer. Validation of the HPTLC method ensures reliability and includes parameters such as linearity, precision, accuracy, specificity, sensitivity, robustness, and limits of detection and quantification. Overall, HPTLC is a powerful tool widely used in pharmaceutical, clinical, and herbal analysis due to its high accuracy, flexibility, and efficiency.</p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_17581599 |
| institution | Zenodo |
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| publishDate | 2025 |
| publisher | Zenodo |
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| spellingShingle | Analytical Method Development and Validation by HPTLC Reshma Lende*, Monali Khatake, Akshada Gadakh TLC, HPTLC, Validation, Method Development <p>High Performance Thin Layer Chromatography (HPTLC) is an advanced form of Thin Layer Chromatography (TLC), used for both qualitative and quantitative analysis of compounds. It offers better separation, sensitivity, and automation compared to traditional TLC. HPTLC works on the principle of differential migration, where substances are separated based on their interaction with the stationary phase (usually silica gel) and a chosen mobile phase (solvent system). Key components of HPTLC instrumentation include an auto-sampler, developing chamber, digital imaging system, and a TLC scanner, all of which improve accuracy and efficiency. The method development process includes steps such as sample preparation, selection of stationary and mobile phases, plate pre-washing, sample application, chromatogram development, and detection. Detection is done using UV light or chemical derivatization, followed by quantification using a densitometer. Validation of the HPTLC method ensures reliability and includes parameters such as linearity, precision, accuracy, specificity, sensitivity, robustness, and limits of detection and quantification. Overall, HPTLC is a powerful tool widely used in pharmaceutical, clinical, and herbal analysis due to its high accuracy, flexibility, and efficiency.</p> |
| title | Analytical Method Development and Validation by HPTLC |
| topic | TLC, HPTLC, Validation, Method Development |
| url | https://doi.org/10.5281/zenodo.17581599 |