| _version_ | 1866901614866989056 |
|---|---|
| author | Dabiri, Damilare Rouse, Warren Moss, Walter |
| author_facet | Dabiri, Damilare Rouse, Warren Moss, Walter |
| contents | <p><em><span>Respiratory Syncytial Virus </span></em><span>Type A (RSV A), a major cause of lower respiratory tract infections in vulnerable populations, is a non-segmented, negative-sense RNA virus with a 15-kilobase genome encoding eleven proteins. RNA secondary structures may be critical for RSV processes like transcription, translation, and genome packaging. This study used a variety of computational tools, including ScanFold and RNAfold, to identify 123 thermodynamically stable RNA structures in the positive-sense antigenome and 113 in the negative-sense genome. Of these 103 and 98 showing structural conservation, respectively. Analysis revealed differential ti/tv ratios between stems and loops, suggesting RNA structural constraints on RSV evolution. Key structures were found in the L (RdRp), Fusion (F), Nonstructural 1 and 2, Matrix 2 (M2), Matrix (M) genes, and the 3' UTR (155 nt Trailer sequence). These findings highlight potentially functional RNA structures that may regulate viral replication, offering novel targets for antiviral drug development</span></p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_17592875 |
| institution | Zenodo |
| language | |
| publishDate | 2025 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | Identification of conserved RNA secondary structures in human Respiratory Syncytial Virus A (hRSV) Dabiri, Damilare Rouse, Warren Moss, Walter <p><em><span>Respiratory Syncytial Virus </span></em><span>Type A (RSV A), a major cause of lower respiratory tract infections in vulnerable populations, is a non-segmented, negative-sense RNA virus with a 15-kilobase genome encoding eleven proteins. RNA secondary structures may be critical for RSV processes like transcription, translation, and genome packaging. This study used a variety of computational tools, including ScanFold and RNAfold, to identify 123 thermodynamically stable RNA structures in the positive-sense antigenome and 113 in the negative-sense genome. Of these 103 and 98 showing structural conservation, respectively. Analysis revealed differential ti/tv ratios between stems and loops, suggesting RNA structural constraints on RSV evolution. Key structures were found in the L (RdRp), Fusion (F), Nonstructural 1 and 2, Matrix 2 (M2), Matrix (M) genes, and the 3' UTR (155 nt Trailer sequence). These findings highlight potentially functional RNA structures that may regulate viral replication, offering novel targets for antiviral drug development</span></p> |
| title | Identification of conserved RNA secondary structures in human Respiratory Syncytial Virus A (hRSV) |
| url | https://doi.org/10.5281/zenodo.17592875 |