Effect of Diazinon on the activity of amino acid in gills and stomach of Channa punctatus
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2025
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| _version_ | 1866901784714280960 |
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| author | Dr Ragini Ahirwar |
| author_facet | Dr Ragini Ahirwar |
| contents | <table style="margin-left: -.25pt; border-collapse: collapse;"> <tbody> <tr style="height: 19.1pt;"> <td style="width: 355.35pt; padding: 0cm 5.4pt 0cm 5.4pt; height: 19.1pt;"> <p><strong><span lang="EN-US">ABSTRACT</span></strong></p> </td> <td style="padding: 0cm 0cm 0cm 0cm;"> <p> </p> </td> </tr> <tr style="height: 91.5pt;"> <td style="width: 361.5pt; padding: 0cm 5.4pt 0cm 5.4pt; height: 91.5pt;" colspan="2"> <p><span lang="EN-US">The extensive use of agricultural pesticides, such as Diazinon, has led to environmental pollution, with residues impacting aquatic ecosystems and food chains. This study investigates the effects of Diazinon on the essential amino acids arginine and tryptophan in the gills and stomach of Channa punctatus. Fish were exposed to 3.1 mg/L Diazinon for one month, with a control group maintained in tap water. Histochemical analyses, including the Sakaguchi reaction for arginine and the DMAB-nitrite method for tryptophan, were performed on gills and stomach tissues. In control fish, strong positive reactions for arginine and tryptophan were observed in gill rakers, adductor and abductor muscles, and stomach mucosa and submucosa, with moderate activity in other tissue layers. Post-Diazinon exposure, a significant reduction or absence of histochemical reactions was noted across all tissues, indicating a depletion of these amino acids. This reduction is likely due to altered membrane permeability caused by Diazinon toxicity. The findings highlight the adverse ecological and physiological impacts of Diazinon on aquatic organisms, emphasizing the need for cautious pesticide use to mitigate environmental and food chain disruptions.</span></p> <p><span lang="EN-US"> </span></p> <p><strong><span lang="EN-US">Key words:</span></strong><span lang="EN-US"><span> </span></span><em><span lang="EN-US">Stomach, Gills, Essential Amino Acid, Arginine and tryptophan</span></em></p> <p></p> <table> <tbody> <tr> <td> <div> <p><strong><span lang="EN-US">Cite This Paper: </span></strong><span lang="EN-US">Dr Ragini Ahirwar (2025). "Effect of Diazinon on the activity of amino acid in gills and stomach of Channa punctatus". <em><span>INTERNATIONAL JOURNAL OF RESEARCH IN ENGINEERING & SCIENCE (IJRES)</span></em>, vol. 9, no. 6, 2025, pp. 58-66. DOI: <a href="https://dx.doi.org/10.5281/zenodo.17599866">https://dx.doi.org/10.5281/zenodo.17599866</a></span></p> </div> </td> </tr> </tbody> </table> <p></p> </td> </tr> </tbody> </table> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_17599866 |
| institution | Zenodo |
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| publishDate | 2025 |
| publisher | Zenodo |
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| spellingShingle | Effect of Diazinon on the activity of amino acid in gills and stomach of Channa punctatus Dr Ragini Ahirwar <table style="margin-left: -.25pt; border-collapse: collapse;"> <tbody> <tr style="height: 19.1pt;"> <td style="width: 355.35pt; padding: 0cm 5.4pt 0cm 5.4pt; height: 19.1pt;"> <p><strong><span lang="EN-US">ABSTRACT</span></strong></p> </td> <td style="padding: 0cm 0cm 0cm 0cm;"> <p> </p> </td> </tr> <tr style="height: 91.5pt;"> <td style="width: 361.5pt; padding: 0cm 5.4pt 0cm 5.4pt; height: 91.5pt;" colspan="2"> <p><span lang="EN-US">The extensive use of agricultural pesticides, such as Diazinon, has led to environmental pollution, with residues impacting aquatic ecosystems and food chains. This study investigates the effects of Diazinon on the essential amino acids arginine and tryptophan in the gills and stomach of Channa punctatus. Fish were exposed to 3.1 mg/L Diazinon for one month, with a control group maintained in tap water. Histochemical analyses, including the Sakaguchi reaction for arginine and the DMAB-nitrite method for tryptophan, were performed on gills and stomach tissues. In control fish, strong positive reactions for arginine and tryptophan were observed in gill rakers, adductor and abductor muscles, and stomach mucosa and submucosa, with moderate activity in other tissue layers. Post-Diazinon exposure, a significant reduction or absence of histochemical reactions was noted across all tissues, indicating a depletion of these amino acids. This reduction is likely due to altered membrane permeability caused by Diazinon toxicity. The findings highlight the adverse ecological and physiological impacts of Diazinon on aquatic organisms, emphasizing the need for cautious pesticide use to mitigate environmental and food chain disruptions.</span></p> <p><span lang="EN-US"> </span></p> <p><strong><span lang="EN-US">Key words:</span></strong><span lang="EN-US"><span> </span></span><em><span lang="EN-US">Stomach, Gills, Essential Amino Acid, Arginine and tryptophan</span></em></p> <p></p> <table> <tbody> <tr> <td> <div> <p><strong><span lang="EN-US">Cite This Paper: </span></strong><span lang="EN-US">Dr Ragini Ahirwar (2025). "Effect of Diazinon on the activity of amino acid in gills and stomach of Channa punctatus". <em><span>INTERNATIONAL JOURNAL OF RESEARCH IN ENGINEERING & SCIENCE (IJRES)</span></em>, vol. 9, no. 6, 2025, pp. 58-66. DOI: <a href="https://dx.doi.org/10.5281/zenodo.17599866">https://dx.doi.org/10.5281/zenodo.17599866</a></span></p> </div> </td> </tr> </tbody> </table> <p></p> </td> </tr> </tbody> </table> |
| title | Effect of Diazinon on the activity of amino acid in gills and stomach of Channa punctatus |
| url | https://doi.org/10.5281/zenodo.17599866 |