ESTIMATION OF A CEFDITOREN FROM A BIOLOGICAL FLUID
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2025
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| author | Ms. Kartiki Kamble Ms. Pranjali Chavan Dr. Paraag Gide |
| author_facet | Ms. Kartiki Kamble Ms. Pranjali Chavan Dr. Paraag Gide |
| contents | <p>Abstract<br>A simple, sensitive, and selective high-performance liquid chromatographic method for the determination of cefditoren in human plasma has been developed. In the presented work, a liquid-liquid extraction reversed-phase high-performance liquid chromatographic (LLE-RP-HPLC) method with UV detection has been validated. The internal standard method was used for quantification and experimental design was used to establish this analytical method. On a reverse phase C18 column, separation was accomplished using an isocratic mobile phase of acetonitrile: water (50:50, v/v). The linear range was estimated to be 1-8 μg/ml. Accuracy and precision both intra-day and inter-day (measured by relative standard deviation, and relative error, respectively) were consistently less than 7% and 5%. The stability of cefditoren was good and stable in human plasma under various conditions. It is suitable for both routine analysis and clinical studies and is cost-effective, simple to use, and has excellent reproducibility.<br>Keywords: Cefditoren, RP-HPLC, Liquid-Liquid Extraction, Validation.</p> |
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| id | zenodo_https___doi_org_10_5281_zenodo_17621167 |
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| publishDate | 2025 |
| publisher | Zenodo |
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| spellingShingle | ESTIMATION OF A CEFDITOREN FROM A BIOLOGICAL FLUID Ms. Kartiki Kamble Ms. Pranjali Chavan Dr. Paraag Gide <p>Abstract<br>A simple, sensitive, and selective high-performance liquid chromatographic method for the determination of cefditoren in human plasma has been developed. In the presented work, a liquid-liquid extraction reversed-phase high-performance liquid chromatographic (LLE-RP-HPLC) method with UV detection has been validated. The internal standard method was used for quantification and experimental design was used to establish this analytical method. On a reverse phase C18 column, separation was accomplished using an isocratic mobile phase of acetonitrile: water (50:50, v/v). The linear range was estimated to be 1-8 μg/ml. Accuracy and precision both intra-day and inter-day (measured by relative standard deviation, and relative error, respectively) were consistently less than 7% and 5%. The stability of cefditoren was good and stable in human plasma under various conditions. It is suitable for both routine analysis and clinical studies and is cost-effective, simple to use, and has excellent reproducibility.<br>Keywords: Cefditoren, RP-HPLC, Liquid-Liquid Extraction, Validation.</p> |
| title | ESTIMATION OF A CEFDITOREN FROM A BIOLOGICAL FLUID |
| url | https://doi.org/10.5281/zenodo.17621167 |