DEVELOPMENT AND VALIDATION OF LLE –HPLC METHOD FOR THE DETERMINATION OF RIFAPENTINE IN HUMAN PLASMA
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2025
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| author | Ms. Pranjali Chavan Ms. Kartiki Kamble Dr. Paraag Gide |
| author_facet | Ms. Pranjali Chavan Ms. Kartiki Kamble Dr. Paraag Gide |
| contents | <p>Abstract<br>A simple, accurate, precise and sensitive reverse phase high performance liquid chromatography method was developed for estimation of rifapentine in human plasma by HPLC method using liquid-liquid extraction (LLE) with UV detection. This analytical method was developed by using experimental design and quantification was accomplished with the internal standard method. No interferences were 0bserved from endogenous compounds of plasma. The accuracy of rifapentine from plasma samples, measured at the three levels of the linear concentration range (1-32 μg/ml) were found between 87 and 110.8% . The intraday and interday precision and accuracy. Mobile phase containing methanol: 20mM phosphate buffer pH (6.5) in ration of 80:20 v/v was used. The flow rate was 1 ml/min with the injection volume of 20μL and effluent was monitored at 478nm. Retention time was 8.3 mins. The method was validated for several parameters like accuracy, linearity, [precision etc as per USFDA guidelines. The values of relative standard deviation and % recovery were found to be satisfactory, indicating that the proposed method is precise and accurate and hence can be used for the routine analysis of rifapentine in human plasma.<br>Keywords: Rifapentine, RP-HPLC, Human Plasma, Liquid-Liquid Extraction & Method Validation.</p> |
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| publishDate | 2025 |
| publisher | Zenodo |
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| spellingShingle | DEVELOPMENT AND VALIDATION OF LLE –HPLC METHOD FOR THE DETERMINATION OF RIFAPENTINE IN HUMAN PLASMA Ms. Pranjali Chavan Ms. Kartiki Kamble Dr. Paraag Gide <p>Abstract<br>A simple, accurate, precise and sensitive reverse phase high performance liquid chromatography method was developed for estimation of rifapentine in human plasma by HPLC method using liquid-liquid extraction (LLE) with UV detection. This analytical method was developed by using experimental design and quantification was accomplished with the internal standard method. No interferences were 0bserved from endogenous compounds of plasma. The accuracy of rifapentine from plasma samples, measured at the three levels of the linear concentration range (1-32 μg/ml) were found between 87 and 110.8% . The intraday and interday precision and accuracy. Mobile phase containing methanol: 20mM phosphate buffer pH (6.5) in ration of 80:20 v/v was used. The flow rate was 1 ml/min with the injection volume of 20μL and effluent was monitored at 478nm. Retention time was 8.3 mins. The method was validated for several parameters like accuracy, linearity, [precision etc as per USFDA guidelines. The values of relative standard deviation and % recovery were found to be satisfactory, indicating that the proposed method is precise and accurate and hence can be used for the routine analysis of rifapentine in human plasma.<br>Keywords: Rifapentine, RP-HPLC, Human Plasma, Liquid-Liquid Extraction & Method Validation.</p> |
| title | DEVELOPMENT AND VALIDATION OF LLE –HPLC METHOD FOR THE DETERMINATION OF RIFAPENTINE IN HUMAN PLASMA |
| url | https://doi.org/10.5281/zenodo.17621271 |