Review on Fluconazole: Properties and Analytical Methods for its Determination

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Autore principale: Dnyaneshwari Gaikwad*, Amol Gayke, Amol Jadhav, Komal Kute
Natura: Recurso digital
Pubblicazione: Zenodo 2025
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author Dnyaneshwari Gaikwad*, Amol Gayke, Amol Jadhav, Komal Kute
author_facet Dnyaneshwari Gaikwad*, Amol Gayke, Amol Jadhav, Komal Kute
contents <p><span>Fluconazole is a triazole antifungal drug widely prescribed for systemic and mucocutaneous fungal infections. It acts by inhibiting fungal cytochrome P450-dependent 14α-demethylase, a key enzyme in ergosterol biosynthesis, leading to disruption of fungal cell membrane integrity. Due to its favorable pharmacokinetic profile, including high oral bioavailability and extensive tissue distribution, Fluconazole remains a first-line antifungal therapy. The accurate determination of Fluconazole in pharmaceutical formulations and biological matrices is critical for quality control, pharmacokinetic, and therapeutic drug monitoring purposes. This review highlights the physicochemical properties, pharmacological profile, and a comprehensive overview of analytical techniques employed for its quantification—such as spectrophotometry, HPLC, LC–MS/MS, GC, CE, and electrochemical methods. Each technique is discussed with respect to its principle, analytical performance, sample preparation, advantages, and limitations. Furthermore, recent advancements in miniaturized and high-sensitivity detection systems are emphasized</span><span>.</span></p>
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spellingShingle Review on Fluconazole: Properties and Analytical Methods for its Determination
Dnyaneshwari Gaikwad*, Amol Gayke, Amol Jadhav, Komal Kute
Fluconazole, Triazole antifungal, HPLC, LC–MS/MS, Spectrophotometry, Analytical methods, Drug quantification, Method validation
<p><span>Fluconazole is a triazole antifungal drug widely prescribed for systemic and mucocutaneous fungal infections. It acts by inhibiting fungal cytochrome P450-dependent 14α-demethylase, a key enzyme in ergosterol biosynthesis, leading to disruption of fungal cell membrane integrity. Due to its favorable pharmacokinetic profile, including high oral bioavailability and extensive tissue distribution, Fluconazole remains a first-line antifungal therapy. The accurate determination of Fluconazole in pharmaceutical formulations and biological matrices is critical for quality control, pharmacokinetic, and therapeutic drug monitoring purposes. This review highlights the physicochemical properties, pharmacological profile, and a comprehensive overview of analytical techniques employed for its quantification—such as spectrophotometry, HPLC, LC–MS/MS, GC, CE, and electrochemical methods. Each technique is discussed with respect to its principle, analytical performance, sample preparation, advantages, and limitations. Furthermore, recent advancements in miniaturized and high-sensitivity detection systems are emphasized</span><span>.</span></p>
title Review on Fluconazole: Properties and Analytical Methods for its Determination
topic Fluconazole, Triazole antifungal, HPLC, LC–MS/MS, Spectrophotometry, Analytical methods, Drug quantification, Method validation
url https://doi.org/10.5281/zenodo.17667963