Enregistré dans:
| Auteurs principaux: | , , , , , , , , , , , , , |
|---|---|
| Format: | Recurso digital |
| Langue: | |
| Publié: |
Zenodo
2020
|
| Accès en ligne: | https://doi.org/10.5281/zenodo.17697108 |
| Tags: |
Ajouter un tag
Pas de tags, Soyez le premier à ajouter un tag!
|
| _version_ | 1866901669233557504 |
|---|---|
| author | Rodriguez-Moncayo, Roberto Cedillo-Alcantar, Diana Fabiola Guevara, Pablo Chavez-Pineda, Oriana Hernández Ortiz, José Antonio Amador Hernandez, Josue Uriel Rojas-Velasco, Gustavo Sanchez-Muñoz, Fausto Manzur Sandoval, Daniel Patino-Lopez, Luis David May-Arrioja, Daniel A. Posadas-Sánchez, Rosalinda Vargas-Alarcon, Gilberto Garcia-Cordero, Jose L |
| author_facet | Rodriguez-Moncayo, Roberto Cedillo-Alcantar, Diana Fabiola Guevara, Pablo Chavez-Pineda, Oriana Hernández Ortiz, José Antonio Amador Hernandez, Josue Uriel Rojas-Velasco, Gustavo Sanchez-Muñoz, Fausto Manzur Sandoval, Daniel Patino-Lopez, Luis David May-Arrioja, Daniel A. Posadas-Sánchez, Rosalinda Vargas-Alarcon, Gilberto Garcia-Cordero, Jose L |
| contents | <p>The applications of serology tests to the virus SARS-CoV-2 are diverse, ranging from diagnosing COVID-19, understanding the humoral response to this disease, estimating its prevalence in a population, to modeling the course of the pandemic. COVID-19 serology assays will significantly benefit from sensitive and reliable technologies that can process dozens of samples in parallel, thus reducing costs and time; however, they will also benefit from biosensors that can assess antibody<br>30 reactivities to multiple SARS-CoV-2 antigens. Here, we report a high-throughput microfluidic device that can assess antibody reactivities against four SARS-CoV-2 antigens from up to 50 sera samples in parallel. This semi-automatic platform measures IgG and IgM levels to four SARS-CoV-2 proteins: the spike protein (S), the S1 subunit (S1), the receptor-binding domain (RBD), and the nucleocapsid (N). After assay optimization, we evaluated sera from infected individuals with COVID-19 and a cohort of archival samples from 2018. The assay achieved a sensitivity of 95% and a specificity of 91%. Nonetheless, both parameters increased to 100% when evaluating sera from individuals in the third week after symptoms onset. To further assess our platform's utility, we monitored the antibody titles from 5 COVID-19 patients over a time course of several weeks. Our platform can aid in global efforts to control and understand COVID-19.</p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_17697108 |
| institution | Zenodo |
| language | |
| publishDate | 2020 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | A high-throughput multiplexed microfluidic device for COVID-19 serology assays Rodriguez-Moncayo, Roberto Cedillo-Alcantar, Diana Fabiola Guevara, Pablo Chavez-Pineda, Oriana Hernández Ortiz, José Antonio Amador Hernandez, Josue Uriel Rojas-Velasco, Gustavo Sanchez-Muñoz, Fausto Manzur Sandoval, Daniel Patino-Lopez, Luis David May-Arrioja, Daniel A. Posadas-Sánchez, Rosalinda Vargas-Alarcon, Gilberto Garcia-Cordero, Jose L <p>The applications of serology tests to the virus SARS-CoV-2 are diverse, ranging from diagnosing COVID-19, understanding the humoral response to this disease, estimating its prevalence in a population, to modeling the course of the pandemic. COVID-19 serology assays will significantly benefit from sensitive and reliable technologies that can process dozens of samples in parallel, thus reducing costs and time; however, they will also benefit from biosensors that can assess antibody<br>30 reactivities to multiple SARS-CoV-2 antigens. Here, we report a high-throughput microfluidic device that can assess antibody reactivities against four SARS-CoV-2 antigens from up to 50 sera samples in parallel. This semi-automatic platform measures IgG and IgM levels to four SARS-CoV-2 proteins: the spike protein (S), the S1 subunit (S1), the receptor-binding domain (RBD), and the nucleocapsid (N). After assay optimization, we evaluated sera from infected individuals with COVID-19 and a cohort of archival samples from 2018. The assay achieved a sensitivity of 95% and a specificity of 91%. Nonetheless, both parameters increased to 100% when evaluating sera from individuals in the third week after symptoms onset. To further assess our platform's utility, we monitored the antibody titles from 5 COVID-19 patients over a time course of several weeks. Our platform can aid in global efforts to control and understand COVID-19.</p> |
| title | A high-throughput multiplexed microfluidic device for COVID-19 serology assays |
| url | https://doi.org/10.5281/zenodo.17697108 |