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Zenodo
2025
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| Accès en ligne: | https://doi.org/10.5281/zenodo.17768817 |
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| _version_ | 1866901197181419520 |
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| author | A.Bharathi K Srikanth |
| author_facet | A.Bharathi K Srikanth |
| contents | <p>Catharanthus roseus (Madagascar periwinkle) is a well-known medicinal plant that produces pharmacologically significant indole alkaloids, particularly vinblastine and vincristine, which are used globally as frontline chemotherapeutics. Despite their clinical importance, the natural yields of these alkaloids in the plant are extremely low, necessitating the development of improved extraction, purification, and semi-synthetic approaches. In this study, we describe a simplified procedure for the extraction of indole alkaloids from the aerial parts of C. roseus, followed by isolation of key monomeric precursors—catharanthine and vindoline. These were subsequently utilized in a semisynthetic coupling strategy to yield vinblastine. Comparative evaluation with conventional Soxhlet and column-based methods demonstrated that the simplified protocol not only reduced solvent and time requirements but also improved the recovery of crude alkaloid fractions. Characterization of intermediates and final vinblastine product was performed using thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), UV–Vis spectrophotometry and Fourier-transform infrared spectroscopy (FTIR). The study highlights the potential of optimized extraction and semi-synthetic approaches for enhancing accessibility to clinically relevant vinca alkaloids. This work has direct implications for industrial production of anticancer agents and provides a scalable framework for future biotechnological interventions.</p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_17768817 |
| institution | Zenodo |
| language | |
| publishDate | 2025 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | Isolation and characterization of Indole Alkaloid from Catharanthus roseus, Plus Semi-Synthesis of Vinblastine A.Bharathi K Srikanth <p>Catharanthus roseus (Madagascar periwinkle) is a well-known medicinal plant that produces pharmacologically significant indole alkaloids, particularly vinblastine and vincristine, which are used globally as frontline chemotherapeutics. Despite their clinical importance, the natural yields of these alkaloids in the plant are extremely low, necessitating the development of improved extraction, purification, and semi-synthetic approaches. In this study, we describe a simplified procedure for the extraction of indole alkaloids from the aerial parts of C. roseus, followed by isolation of key monomeric precursors—catharanthine and vindoline. These were subsequently utilized in a semisynthetic coupling strategy to yield vinblastine. Comparative evaluation with conventional Soxhlet and column-based methods demonstrated that the simplified protocol not only reduced solvent and time requirements but also improved the recovery of crude alkaloid fractions. Characterization of intermediates and final vinblastine product was performed using thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), UV–Vis spectrophotometry and Fourier-transform infrared spectroscopy (FTIR). The study highlights the potential of optimized extraction and semi-synthetic approaches for enhancing accessibility to clinically relevant vinca alkaloids. This work has direct implications for industrial production of anticancer agents and provides a scalable framework for future biotechnological interventions.</p> |
| title | Isolation and characterization of Indole Alkaloid from Catharanthus roseus, Plus Semi-Synthesis of Vinblastine |
| url | https://doi.org/10.5281/zenodo.17768817 |