Adaptation of Pseudomonas aeruginosa to the lung allograft environment in lung transplant patients
Fuente:
Zenodo
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Recurso digital |
| Sprache: | Englisch |
| Veröffentlicht: |
Zenodo
2026
|
| Schlagworte: | |
| Online-Zugang: | |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| _version_ | 1866901897247457280 |
|---|---|
| author | Köhler, Thilo |
| author_facet | Köhler, Thilo |
| contents | <p><span lang="EN-GB">Lung transplantation (LT) is the ultimate treatment option for patients suffering from end stage cystic fibrosis (CF) or chronic obstructive lung diseases. Most LT-patients, colonized pre-LT by <em>Pseudomonas aeruginosa</em> witness colonization of their non-CF allograft within a few days or weeks post-LT, thereby compromising graft and life expectancy. How <em>P. aeruginosa</em> isolates adapted for years to the specific CF lung environment efficiently colonize and survive in the non-CF allograft environment remains unclear. To address this question, we collected sequential isolates from CF LT-recipients and non-CF LT control patients and performed phenotypic and genetic analyses of pairs of early and late isolates from LT-patients. We found evidence for mutations compatible with a switch from biofilm to planktonic lifestyle as well as loss of mucoid phenotypes. Hypermutators, characteristic of chronic CF-adapted isolates, were also found in four LT-patients. Their persistence in the non-CF allograft environment suggests a continuous seeding from the sinuses. Our results suggest that in CF LT-recipients efficient colonisation by <em>P. aeruginosa</em> of the allograft implies both adaptation and continuous seeding from the sinuses to the lower respiratory tract.</span></p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_17779534 |
| institution | Zenodo |
| language | eng |
| publishDate | 2026 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | Adaptation of Pseudomonas aeruginosa to the lung allograft environment in lung transplant patients Köhler, Thilo Pseudomonas aeruginosa Lung Transplantation <p><span lang="EN-GB">Lung transplantation (LT) is the ultimate treatment option for patients suffering from end stage cystic fibrosis (CF) or chronic obstructive lung diseases. Most LT-patients, colonized pre-LT by <em>Pseudomonas aeruginosa</em> witness colonization of their non-CF allograft within a few days or weeks post-LT, thereby compromising graft and life expectancy. How <em>P. aeruginosa</em> isolates adapted for years to the specific CF lung environment efficiently colonize and survive in the non-CF allograft environment remains unclear. To address this question, we collected sequential isolates from CF LT-recipients and non-CF LT control patients and performed phenotypic and genetic analyses of pairs of early and late isolates from LT-patients. We found evidence for mutations compatible with a switch from biofilm to planktonic lifestyle as well as loss of mucoid phenotypes. Hypermutators, characteristic of chronic CF-adapted isolates, were also found in four LT-patients. Their persistence in the non-CF allograft environment suggests a continuous seeding from the sinuses. Our results suggest that in CF LT-recipients efficient colonisation by <em>P. aeruginosa</em> of the allograft implies both adaptation and continuous seeding from the sinuses to the lower respiratory tract.</span></p> |
| title | Adaptation of Pseudomonas aeruginosa to the lung allograft environment in lung transplant patients |
| topic | Pseudomonas aeruginosa Lung Transplantation |
| url | https://doi.org/10.5281/zenodo.17779534 |