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2025
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| Online Access: | https://doi.org/10.5281/zenodo.17788185 |
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| author | Emilio PARISINI |
| author_facet | Emilio PARISINI |
| contents | Diabetes is a chronic medical condition characterized by high blood sugar levels. The worldwide incidence of this pathology is booming in modern society, with a forecast increase of 20% in patients in 2030 and with one in two diabetic adults still going undiagnosed. The monitoring of blood sugar levels has become an essential practice in the diagnosis of this pathology. Relative to direct glucose monitoring, the measurement of the level of glycated proteins in the blood (such as glycated haemoglobin, HbA1c) is a recommended practice, as it is less subject to fluctuations and therefore more reliable. Fructosyl Peptide Oxidase (FPOX) is a promising class of enzymes for the monitoring of glycated proteins in the blood. However, due to steric hindrance, currently available enzymes are inactive on whole glycated proteins, and their use requires a preliminary proteolytic digestion of the sample. This complicates the measurement process, as it can only be performed by qualified personnel. A strip-based test, similar to currently available sensors for glucose monitoring, is still not available for HbA1c. This project aims to apply a rational design approach to the engineering of new FPOX enzymes with a wider access tunnel to the catalytic site, to process whole glycated proteins. Moreover, a new immobilization protocol will be developed to obtain an FPOX-enzyme-based bio-hybrid material that can facilitate the development of an easy-to-use self-care diabetes monitoring device. |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_17788185 |
| institution | Zenodo |
| language | |
| publishDate | 2025 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | Exploring enzyme-based biohybrid materials as platforms for diabetes monitoring applications Emilio PARISINI Diabetes is a chronic medical condition characterized by high blood sugar levels. The worldwide incidence of this pathology is booming in modern society, with a forecast increase of 20% in patients in 2030 and with one in two diabetic adults still going undiagnosed. The monitoring of blood sugar levels has become an essential practice in the diagnosis of this pathology. Relative to direct glucose monitoring, the measurement of the level of glycated proteins in the blood (such as glycated haemoglobin, HbA1c) is a recommended practice, as it is less subject to fluctuations and therefore more reliable. Fructosyl Peptide Oxidase (FPOX) is a promising class of enzymes for the monitoring of glycated proteins in the blood. However, due to steric hindrance, currently available enzymes are inactive on whole glycated proteins, and their use requires a preliminary proteolytic digestion of the sample. This complicates the measurement process, as it can only be performed by qualified personnel. A strip-based test, similar to currently available sensors for glucose monitoring, is still not available for HbA1c. This project aims to apply a rational design approach to the engineering of new FPOX enzymes with a wider access tunnel to the catalytic site, to process whole glycated proteins. Moreover, a new immobilization protocol will be developed to obtain an FPOX-enzyme-based bio-hybrid material that can facilitate the development of an easy-to-use self-care diabetes monitoring device. |
| title | Exploring enzyme-based biohybrid materials as platforms for diabetes monitoring applications |
| url | https://doi.org/10.5281/zenodo.17788185 |