Impaired nitric oxide-dependent endothelial function in young male individualswith obesity before the onset of symptoms and complications
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2025
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| _version_ | 1866901090580037632 |
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| author | Sartorio, Alessandro |
| author_facet | Sartorio, Alessandro |
| contents | <p>Endothelial dysfunction drives obesity-related complications. Doppler ultrasound measurement of blood flow during 1-min passive leg movements (PLM) is a valuable non-invasive tool for assessing endothelial function and nitric oxide (NO)-mediated<br>vasodilation. The objectives of this work were t o identify endothelial dysfunction biomarkers in young individuals with obesity (OB) using the PLM test; to evaluate the effects of a rehabilitation programme on these biomarkers; and to explore associations between PLM data, oxidative metabolism and blood biomarkers of microvascular impairment. Fifteen male OB (age 17 ± 4 years; body mass 121.4 ± 24.1 kg; body mass index 39.3 ± 7.5 kg m−2) were tested before (PRE) and after (POST) a 3-week multidisciplinary body mass reduction programme. Fifteen age-matched normal-weight males (CTRL) underwent PRE measurements. Participants performed an incremental exercise, a PLM test and underwent blood biomarker analysis. Peak<br>oxygen uptake and ventilatory thresholds (mL kg−1 min−1) were ∼40% lower in OB versus CTRL (P < 0.001) and improved by ∼8% in OB POST versus PRE (P < 0.05). Plasma nitrite concentration was lower in OB (0.18 ± 0.09 μmol L−1) versus CTRL<br>(0.51 ± 0.49; P = 0.02). Baseline blood flow, normalized for appendicular muscle mass, was similar between the two groups, whereas peak blood flow, Δpeak (difference between peak and baseline) and the area under the blood flow versus time curve<br>were significantly lower in OB PRE, with improvements in OB POST. Several blood biomarkers of endothelial barrier function and permeability differed in OB versus CTRL. The blunted PLM-induced hyperaemic response and lower plasma nitrite levels<br>indicate impaired endothelial function in young individuals with obesity, occurring before the onset of cardiovascular and metabolic complications.</p> <p><span>These data cannot be made publicly available as they include sensitive information, but they can be made available upon reasonable request of interested researchers to the corresponding author, who will forward a data transfer agreement request to the relevant Ethical Committee. Requests can be addressed to Dr. Alessandro Sartorio (sartorio@auxologico.it).</span></p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_17803374 |
| institution | Zenodo |
| language | |
| publishDate | 2025 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | Impaired nitric oxide-dependent endothelial function in young male individualswith obesity before the onset of symptoms and complications Sartorio, Alessandro Exercise tolerance Endothelial function Nitric oxide Obesity PLM <p>Endothelial dysfunction drives obesity-related complications. Doppler ultrasound measurement of blood flow during 1-min passive leg movements (PLM) is a valuable non-invasive tool for assessing endothelial function and nitric oxide (NO)-mediated<br>vasodilation. The objectives of this work were t o identify endothelial dysfunction biomarkers in young individuals with obesity (OB) using the PLM test; to evaluate the effects of a rehabilitation programme on these biomarkers; and to explore associations between PLM data, oxidative metabolism and blood biomarkers of microvascular impairment. Fifteen male OB (age 17 ± 4 years; body mass 121.4 ± 24.1 kg; body mass index 39.3 ± 7.5 kg m−2) were tested before (PRE) and after (POST) a 3-week multidisciplinary body mass reduction programme. Fifteen age-matched normal-weight males (CTRL) underwent PRE measurements. Participants performed an incremental exercise, a PLM test and underwent blood biomarker analysis. Peak<br>oxygen uptake and ventilatory thresholds (mL kg−1 min−1) were ∼40% lower in OB versus CTRL (P < 0.001) and improved by ∼8% in OB POST versus PRE (P < 0.05). Plasma nitrite concentration was lower in OB (0.18 ± 0.09 μmol L−1) versus CTRL<br>(0.51 ± 0.49; P = 0.02). Baseline blood flow, normalized for appendicular muscle mass, was similar between the two groups, whereas peak blood flow, Δpeak (difference between peak and baseline) and the area under the blood flow versus time curve<br>were significantly lower in OB PRE, with improvements in OB POST. Several blood biomarkers of endothelial barrier function and permeability differed in OB versus CTRL. The blunted PLM-induced hyperaemic response and lower plasma nitrite levels<br>indicate impaired endothelial function in young individuals with obesity, occurring before the onset of cardiovascular and metabolic complications.</p> <p><span>These data cannot be made publicly available as they include sensitive information, but they can be made available upon reasonable request of interested researchers to the corresponding author, who will forward a data transfer agreement request to the relevant Ethical Committee. Requests can be addressed to Dr. Alessandro Sartorio (sartorio@auxologico.it).</span></p> |
| title | Impaired nitric oxide-dependent endothelial function in young male individualswith obesity before the onset of symptoms and complications |
| topic | Exercise tolerance Endothelial function Nitric oxide Obesity PLM |
| url | https://doi.org/10.5281/zenodo.17803374 |