Hepatoprotective Effects of Aqueous Extract of Citrullus lanatus Seed on Alcohol-Induced Liver Toxicity in Wistar Rats

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Autori principali: Afuberoh Francis Chukwudi, Eyeghre Onoriode Akpoghene, Anyaogu Charles Chinemeze, Okoye Ogochukwu Fidelis, Darlington Victor Nweze, Okonkwo Ifeoma Rita, Akpudo Genevieve Chiemelie
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author Afuberoh Francis Chukwudi
Eyeghre Onoriode Akpoghene
Anyaogu Charles Chinemeze
Okoye Ogochukwu Fidelis
Darlington Victor Nweze
Okonkwo Ifeoma Rita
Akpudo Genevieve Chiemelie
author_facet Afuberoh Francis Chukwudi
Eyeghre Onoriode Akpoghene
Anyaogu Charles Chinemeze
Okoye Ogochukwu Fidelis
Darlington Victor Nweze
Okonkwo Ifeoma Rita
Akpudo Genevieve Chiemelie
contents <p><strong><span lang="EN-US">Abstract:</span></strong><span lang="EN-US"> Chronic alcohol consumption leads to oxidative stress and hepatic dysfunction. Natural antioxidants from plant sources such as <em>Citrullus</em> <em>lanatus</em> (watermelon) seeds may protect against such damage through free radical scavenging and membrane-stabilizing actions (Osna <em>et al</em>., 2017; Harjumäki <em>et al</em>., 2021). This study evaluated the hepatoprotective potential of aqueous extract of <em>Citrullus lanatus</em> seed (AECL) on alcohol-induced liver toxicity in Wistar rats. Thirty adult male Wistar rats (130–150 g) were divided into five groups (n = 6): Group I (normal control) received feed and water only; Group II (alcohol control) received 1 mL ethanol daily for 2 weeks; Group III (extract control) received AECL 500 mg/kg only; Group IV received ethanol + AECL 500 mg/kg; and Group V received ethanol + AECL 1000 mg/kg. Treatments were given orally for 6 weeks. Serum ALT, AST, ALP, and total protein were assayed. Data were analyzed using one-way ANOVA with LSD post-hoc test (p ≤ 0.05). Alcohol exposure significantly increased (p < 0.05) ALT, AST, and ALP, while total protein decreased. Co-administration of AECL (500 mg/kg and 1000 mg/kg) significantly reversed these alterations toward normal. The higher dose produced effects comparable to control. Aqueous extract of <em>Citrullus</em> <em>lanatus</em> seed protects against alcohol-induced liver injury, likely via antioxidant and membrane-stabilizing mechanisms.</span></p> <p><strong><span lang="EN-US">Keywords:</span></strong><span lang="EN-US"> <em>Citrullus lanatus</em>, hepatotoxicity, ethanol, liver enzymes, antioxidant, Wistar rat.</span></p> <p><strong><span lang="EN-US">Title:</span></strong><span lang="EN-US"> Hepatoprotective Effects of Aqueous Extract of Citrullus lanatus Seed on Alcohol-Induced Liver Toxicity in Wistar Rats</span></p> <p><strong><span lang="EN-US">Author:</span></strong><span lang="EN-US"> Afuberoh Francis Chukwudi, Eyeghre Onoriode Akpoghene, Anyaogu Charles Chinemeze, Okoye Ogochukwu Fidelis, Darlington Victor Nweze, Okonkwo Ifeoma Rita, Akpudo Genevieve Chiemelie</span></p> <p><strong><span lang="EN-US">International Journal of Recent Research in Life Sciences (IJRRLS)</span></strong></p> <p><strong><span lang="EN-US">ISSN 2349-7823</span></strong></p> <p><strong><span lang="EN-US">Vol. 12, Issue 4, October 2025 - December 2025</span></strong></p> <p><strong><span lang="EN-US">Page No: 37-43</span></strong></p> <p><strong><span lang="EN-US">Paper Publications</span></strong></p> <p><strong><span lang="EN-US">Website: www.paperpublications.org</span></strong></p> <p><strong><span lang="EN-US">Published Date: 17-December-2025</span></strong></p> <p><strong><span lang="EN-US">DOI: <a href="https://doi.org/10.5281/zenodo.17961428">https://doi.org/10.5281/zenodo.17961428</a></span></strong></p> <p><strong><span lang="EN-US">Paper Download Link (Source)</span></strong></p> <p><strong><span lang="EN-US"><a href="https://www.paperpublications.org/upload/book/Hepatoprotective%20Effects%20of%20Aqueous%20Extract-17122025-2.pdf">https://www.paperpublications.org/upload/book/Hepatoprotective%20Effects%20of%20Aqueous%20Extract-17122025-2.pdf</a></span></strong></p>
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spellingShingle Hepatoprotective Effects of Aqueous Extract of Citrullus lanatus Seed on Alcohol-Induced Liver Toxicity in Wistar Rats
Afuberoh Francis Chukwudi
Eyeghre Onoriode Akpoghene
Anyaogu Charles Chinemeze
Okoye Ogochukwu Fidelis
Darlington Victor Nweze
Okonkwo Ifeoma Rita
Akpudo Genevieve Chiemelie
Citrullus lanatus
hepatotoxicity
ethanol
liver enzymes
antioxidant
Wistar rat
<p><strong><span lang="EN-US">Abstract:</span></strong><span lang="EN-US"> Chronic alcohol consumption leads to oxidative stress and hepatic dysfunction. Natural antioxidants from plant sources such as <em>Citrullus</em> <em>lanatus</em> (watermelon) seeds may protect against such damage through free radical scavenging and membrane-stabilizing actions (Osna <em>et al</em>., 2017; Harjumäki <em>et al</em>., 2021). This study evaluated the hepatoprotective potential of aqueous extract of <em>Citrullus lanatus</em> seed (AECL) on alcohol-induced liver toxicity in Wistar rats. Thirty adult male Wistar rats (130–150 g) were divided into five groups (n = 6): Group I (normal control) received feed and water only; Group II (alcohol control) received 1 mL ethanol daily for 2 weeks; Group III (extract control) received AECL 500 mg/kg only; Group IV received ethanol + AECL 500 mg/kg; and Group V received ethanol + AECL 1000 mg/kg. Treatments were given orally for 6 weeks. Serum ALT, AST, ALP, and total protein were assayed. Data were analyzed using one-way ANOVA with LSD post-hoc test (p ≤ 0.05). Alcohol exposure significantly increased (p < 0.05) ALT, AST, and ALP, while total protein decreased. Co-administration of AECL (500 mg/kg and 1000 mg/kg) significantly reversed these alterations toward normal. The higher dose produced effects comparable to control. Aqueous extract of <em>Citrullus</em> <em>lanatus</em> seed protects against alcohol-induced liver injury, likely via antioxidant and membrane-stabilizing mechanisms.</span></p> <p><strong><span lang="EN-US">Keywords:</span></strong><span lang="EN-US"> <em>Citrullus lanatus</em>, hepatotoxicity, ethanol, liver enzymes, antioxidant, Wistar rat.</span></p> <p><strong><span lang="EN-US">Title:</span></strong><span lang="EN-US"> Hepatoprotective Effects of Aqueous Extract of Citrullus lanatus Seed on Alcohol-Induced Liver Toxicity in Wistar Rats</span></p> <p><strong><span lang="EN-US">Author:</span></strong><span lang="EN-US"> Afuberoh Francis Chukwudi, Eyeghre Onoriode Akpoghene, Anyaogu Charles Chinemeze, Okoye Ogochukwu Fidelis, Darlington Victor Nweze, Okonkwo Ifeoma Rita, Akpudo Genevieve Chiemelie</span></p> <p><strong><span lang="EN-US">International Journal of Recent Research in Life Sciences (IJRRLS)</span></strong></p> <p><strong><span lang="EN-US">ISSN 2349-7823</span></strong></p> <p><strong><span lang="EN-US">Vol. 12, Issue 4, October 2025 - December 2025</span></strong></p> <p><strong><span lang="EN-US">Page No: 37-43</span></strong></p> <p><strong><span lang="EN-US">Paper Publications</span></strong></p> <p><strong><span lang="EN-US">Website: www.paperpublications.org</span></strong></p> <p><strong><span lang="EN-US">Published Date: 17-December-2025</span></strong></p> <p><strong><span lang="EN-US">DOI: <a href="https://doi.org/10.5281/zenodo.17961428">https://doi.org/10.5281/zenodo.17961428</a></span></strong></p> <p><strong><span lang="EN-US">Paper Download Link (Source)</span></strong></p> <p><strong><span lang="EN-US"><a href="https://www.paperpublications.org/upload/book/Hepatoprotective%20Effects%20of%20Aqueous%20Extract-17122025-2.pdf">https://www.paperpublications.org/upload/book/Hepatoprotective%20Effects%20of%20Aqueous%20Extract-17122025-2.pdf</a></span></strong></p>
title Hepatoprotective Effects of Aqueous Extract of Citrullus lanatus Seed on Alcohol-Induced Liver Toxicity in Wistar Rats
topic Citrullus lanatus
hepatotoxicity
ethanol
liver enzymes
antioxidant
Wistar rat
url https://doi.org/10.5281/zenodo.17961428