Development of HPLC method for estimation of β-sitosterol from Peliosanthes micrantha rhizomes

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Main Authors: Thuy, Do Ngoc, Hung, Le Ngoc, Huong, Nguyen Thi
Format: Recurso digital
Language:English
Published: Zenodo 2025
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author Thuy, Do Ngoc
Hung, Le Ngoc
Huong, Nguyen Thi
author_facet Thuy, Do Ngoc
Hung, Le Ngoc
Huong, Nguyen Thi
contents <p><span lang="EN-US">The medicinal plant <em>Peliosanthes micrantha</em> of Vietnam has been studied recently. Two</span><span lang="EN-US"> </span><span lang="EN-US">components, one of which was β-sitosterol</span><span lang="EN-US">, were isolated. </span><em><span lang="EN-US">P. micrantha</span></em><span lang="EN-US"> rhizomes were first extracted by ethanol (PM-R-ET)</span><span lang="VI">. </span><span lang="EN-US">β-sitosterol</span><span lang="EN-US"> was then</span><span lang="VI"> isolated </span><span lang="EN-US">as a standard compound. High-performance liquid chromatography method (HPLC) was validated and used for the quantification of </span><span lang="EN-US">β-sitosterol</span><span lang="EN-US"> in the </span><span lang="VI">extract</span><span lang="EN-US">. We concluded in this study that </span><span lang="EN-US">β-sitosterol content was </span><span lang="EN-US">significant at 0.71 mg/g of fresh <em>P. micrantha</em> rhizome</span><span lang="VI"> extract.</span></p>
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id zenodo_https___doi_org_10_5281_zenodo_18060137
institution Zenodo
language eng
publishDate 2025
publisher Zenodo
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spellingShingle Development of HPLC method for estimation of β-sitosterol from Peliosanthes micrantha rhizomes
Thuy, Do Ngoc
Hung, Le Ngoc
Huong, Nguyen Thi
Peliosanthes micrantha, rhizomes, ethanol, β-sitosterol standard, quantification, HPLC
<p><span lang="EN-US">The medicinal plant <em>Peliosanthes micrantha</em> of Vietnam has been studied recently. Two</span><span lang="EN-US"> </span><span lang="EN-US">components, one of which was β-sitosterol</span><span lang="EN-US">, were isolated. </span><em><span lang="EN-US">P. micrantha</span></em><span lang="EN-US"> rhizomes were first extracted by ethanol (PM-R-ET)</span><span lang="VI">. </span><span lang="EN-US">β-sitosterol</span><span lang="EN-US"> was then</span><span lang="VI"> isolated </span><span lang="EN-US">as a standard compound. High-performance liquid chromatography method (HPLC) was validated and used for the quantification of </span><span lang="EN-US">β-sitosterol</span><span lang="EN-US"> in the </span><span lang="VI">extract</span><span lang="EN-US">. We concluded in this study that </span><span lang="EN-US">β-sitosterol content was </span><span lang="EN-US">significant at 0.71 mg/g of fresh <em>P. micrantha</em> rhizome</span><span lang="VI"> extract.</span></p>
title Development of HPLC method for estimation of β-sitosterol from Peliosanthes micrantha rhizomes
topic Peliosanthes micrantha, rhizomes, ethanol, β-sitosterol standard, quantification, HPLC
url https://doi.org/10.5281/zenodo.18060137