Formulation and Evaluation of Herbal Anti-Inflammatory Cream from Aegle Marmelos

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Main Author: Vasudha Mane*, Payal Mudhe, Divya Taral, Mitesh Patil, Umesh Kamble
Format: Recurso digital
Published: Zenodo 2025
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author Vasudha Mane*, Payal Mudhe, Divya Taral, Mitesh Patil, Umesh Kamble
author_facet Vasudha Mane*, Payal Mudhe, Divya Taral, Mitesh Patil, Umesh Kamble
contents <p><span>Herbal-based topical formulations are increasingly recognized as safer and more biocompatible alternatives to synthetic anti-inflammatory agents. In line with this growing interest, the present study focuses on the development of a naturally derived anti-inflammatory cream utilizing the extract of Aegle marmelos, a medicinal plant widely valued in traditional systems for its therapeutic potential. This research highlights the importance of incorporating phytochemical-rich herbal ingredients into modern topical delivery systems to achieve effective, stable, and skin-friendly formulations.In this study, Aegle marmelos leaves were collected, processed, and subjected to Soxhlet extraction using eth.anol as the solvent. Phytochemical screening of the extract confirmed the presence of key bioactive compounds such as flavonoids, phenolics, tannins, alkaloids, saponins, glycosides, and terpenoids—constituents known to exhibit potent anti-inflammatory and antioxidant properties. A herbal cream formulation (F1) was successfully prepared as an oil-in-water (O/W) emulsion using optimized conditions and a combination of pharmaceutical excipients including stearic acid, cetostearyl alcohol, beeswax, coconut oil, glycerin, and triethanolamine.The prepared formulation was subjected to comprehensive evaluation, covering organoleptic characteristics, physicochemical parameters, physical stability, skin safety, and therapeutic efficacy. The cream displayed desirable properties such as smooth consistency, homogeneous appearance, pleasant herbal odor, and a skin-compatible pH of 6.7. It demonstrated excellent spreadability, easy washability, and no signs of phase separation. Stability studies conducted over 60 days further confirmed the robustness and shelf-life potential of the formulation, showing no significant change in pH, color, consistency, or texture. Skin irritation studies verified that the cream was non-irritant and safe for topical application.The in vitro anti-inflammatory activity, assessed using the protein denaturation method, revealed significant inhibition, thereby confirming the therapeutic effectiveness of the Aegle marmelos extract.Overall, the findings establish that the developed herbal anti-inflammatory cream is safe, stable, non-irritant, and pharmaceutically acceptable. The study strongly validates the use of Aegle marmelos as an effective natural alternative for topical management of inflammation and provides a promising foundation for future herbal-based dermatological formulations.</span></p>
format Recurso digital
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publishDate 2025
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spellingShingle Formulation and Evaluation of Herbal Anti-Inflammatory Cream from Aegle Marmelos
Vasudha Mane*, Payal Mudhe, Divya Taral, Mitesh Patil, Umesh Kamble
Aegle marmelos, herbal cream, anti-inflammatory, topical formulation, phytochemicals, Soxhlet extraction, oil-in-water emulsion, stability, skin safety, protein denaturation assay.
<p><span>Herbal-based topical formulations are increasingly recognized as safer and more biocompatible alternatives to synthetic anti-inflammatory agents. In line with this growing interest, the present study focuses on the development of a naturally derived anti-inflammatory cream utilizing the extract of Aegle marmelos, a medicinal plant widely valued in traditional systems for its therapeutic potential. This research highlights the importance of incorporating phytochemical-rich herbal ingredients into modern topical delivery systems to achieve effective, stable, and skin-friendly formulations.In this study, Aegle marmelos leaves were collected, processed, and subjected to Soxhlet extraction using eth.anol as the solvent. Phytochemical screening of the extract confirmed the presence of key bioactive compounds such as flavonoids, phenolics, tannins, alkaloids, saponins, glycosides, and terpenoids—constituents known to exhibit potent anti-inflammatory and antioxidant properties. A herbal cream formulation (F1) was successfully prepared as an oil-in-water (O/W) emulsion using optimized conditions and a combination of pharmaceutical excipients including stearic acid, cetostearyl alcohol, beeswax, coconut oil, glycerin, and triethanolamine.The prepared formulation was subjected to comprehensive evaluation, covering organoleptic characteristics, physicochemical parameters, physical stability, skin safety, and therapeutic efficacy. The cream displayed desirable properties such as smooth consistency, homogeneous appearance, pleasant herbal odor, and a skin-compatible pH of 6.7. It demonstrated excellent spreadability, easy washability, and no signs of phase separation. Stability studies conducted over 60 days further confirmed the robustness and shelf-life potential of the formulation, showing no significant change in pH, color, consistency, or texture. Skin irritation studies verified that the cream was non-irritant and safe for topical application.The in vitro anti-inflammatory activity, assessed using the protein denaturation method, revealed significant inhibition, thereby confirming the therapeutic effectiveness of the Aegle marmelos extract.Overall, the findings establish that the developed herbal anti-inflammatory cream is safe, stable, non-irritant, and pharmaceutically acceptable. The study strongly validates the use of Aegle marmelos as an effective natural alternative for topical management of inflammation and provides a promising foundation for future herbal-based dermatological formulations.</span></p>
title Formulation and Evaluation of Herbal Anti-Inflammatory Cream from Aegle Marmelos
topic Aegle marmelos, herbal cream, anti-inflammatory, topical formulation, phytochemicals, Soxhlet extraction, oil-in-water emulsion, stability, skin safety, protein denaturation assay.
url https://doi.org/10.5281/zenodo.18106701