Advances and Challenges in Diagnostic Approaches to Human Brucellosis: A Comprehensive Review
Fuente:
Zenodo
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Recurso digital |
| Veröffentlicht: |
Zenodo
2025
|
| Online-Zugang: | |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| _version_ | 1866902093659373568 |
|---|---|
| author | Dr. Jinal Patel |
| author_facet | Dr. Jinal Patel |
| contents | <p>Brucellosis remains a significant zoonotic infection worldwide, particularly in developing countries where livestock farming is prevalent. Accurate and timely diagnosis of brucellosis is crucial to prevent chronic complications and reduce community spread. This review explores traditional and modern diagnostic methods used for human brucellosis, summarizing serological, microbiological, and molecular techniques. Conventional serological assays such as Rose Bengal Plate Test and Serum Agglutination Test, though widely used, often lack specificity in endemic areas. Culture remains the gold standard but is limited by its low sensitivity and biohazard concerns. Polymerase chain reaction (PCR)-based assays and newer molecular techniques offer promising sensitivity and specificity but face practical barriers in low-resource settings. We also discuss emerging diagnostic approaches, including proteomic and metabolomic methods. The literature search was conducted using PubMed, Scopus, and Google Scholar databases from 2000 to 2025. Considered WHO and OIE reports for global perspectives. This review aims to provide clinicians and public health professionals with an updated understanding of brucellosis diagnostics to aid timely decision-making. Further advancements in point-of-care assays and harmonization of molecular methods are essential to improve detection rates, especially in endemic regions.</p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_18153417 |
| institution | Zenodo |
| language | |
| publishDate | 2025 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | Advances and Challenges in Diagnostic Approaches to Human Brucellosis: A Comprehensive Review Dr. Jinal Patel <p>Brucellosis remains a significant zoonotic infection worldwide, particularly in developing countries where livestock farming is prevalent. Accurate and timely diagnosis of brucellosis is crucial to prevent chronic complications and reduce community spread. This review explores traditional and modern diagnostic methods used for human brucellosis, summarizing serological, microbiological, and molecular techniques. Conventional serological assays such as Rose Bengal Plate Test and Serum Agglutination Test, though widely used, often lack specificity in endemic areas. Culture remains the gold standard but is limited by its low sensitivity and biohazard concerns. Polymerase chain reaction (PCR)-based assays and newer molecular techniques offer promising sensitivity and specificity but face practical barriers in low-resource settings. We also discuss emerging diagnostic approaches, including proteomic and metabolomic methods. The literature search was conducted using PubMed, Scopus, and Google Scholar databases from 2000 to 2025. Considered WHO and OIE reports for global perspectives. This review aims to provide clinicians and public health professionals with an updated understanding of brucellosis diagnostics to aid timely decision-making. Further advancements in point-of-care assays and harmonization of molecular methods are essential to improve detection rates, especially in endemic regions.</p> |
| title | Advances and Challenges in Diagnostic Approaches to Human Brucellosis: A Comprehensive Review |
| url | https://doi.org/10.5281/zenodo.18153417 |