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| Format: | Recurso digital |
| Language: | English |
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2025
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| Online Access: | https://doi.org/10.5281/zenodo.18172770 |
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| _version_ | 1866901234758189056 |
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| author | Adeoye, O. J |
| author_facet | Adeoye, O. J |
| contents | <p><span lang="EN">Carrot (<em>Daucus carota</em> L.) is a vegetable that is mostly eaten raw without cooking. It therefore serves as a vehicle for fungi to move from farm to stomach, these microflora are also responsible for carrot spoilage leading to losses. In this study, fungi the causing diseases in carrot production were investigated. Carrots were purchased from local farmers in Akure, Ondo State, Nigeria, labeled appropriately and taken to the laboratory for analyses. The carrots were divided into 2 groups (A and B). Group A was further divided into subgroups and kept in refrigerator (4 ± 2 <sup>o</sup>C) and colanders (30 ± 2 <sup>o</sup>C) to study the fungi responsible for its spoilage, group B was used to determine the fungi present on carrot. The fungi present on the surface of spoilt carrots and fresh carrots were isolated by scraping the surface of the carrot with sterile knife on sterile alluminium foil. One gram of the scrapped carrot surface was weighed aseptically and diluted serially to dilution 10<sup>-3</sup>. Saboraud agar (SA) and potato dextrose agar (PDA) were used for the culturing, and incubated at 28 <sup>o</sup>C for 72 hours. The morphology of the pure fungus on plate was observed<strong> </strong>for its shape, colour (front and reverse) under the microscope using x10 and x40 objectives. <em>Sacharomyces cerevisiae</em>,<em> Aspergillus nidulans</em>,<em> Pleurothecium recurvatum </em>and<em> Rhizopus nigricans</em> were the fungi isolated from the surface of fresh carrots while (<em>Sclerotini sclerotiorum</em>,<em> Penicillium natatum</em>,<em> Aspergillus niger </em>and<em> Rhizopus nigricans)<strong> </strong></em>were isolated from spoilt carrot. Some of the fungi found on the fresh carrots are pathogenic, fungal species like <em>A. niger</em> could produce toxic metabolites in the affected sites, constituting a potential health hazard for humans, <em>Sclerotini sclerotiorum</em> causes devastating losses to farmers. It is therefore important to look for means of inhibiting the growth of these fungi on carrots to prevent food borne diseases and loss of farm produce. </span></p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_18172770 |
| institution | Zenodo |
| language | eng |
| publishDate | 2025 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | Fungal Pathogens Responsible for Diseases in Carrot (Daucus Carota l.) Production in Ondo State Nigeria Adeoye, O. J Food-borne diseases food spoilage microflora carrots fungi <p><span lang="EN">Carrot (<em>Daucus carota</em> L.) is a vegetable that is mostly eaten raw without cooking. It therefore serves as a vehicle for fungi to move from farm to stomach, these microflora are also responsible for carrot spoilage leading to losses. In this study, fungi the causing diseases in carrot production were investigated. Carrots were purchased from local farmers in Akure, Ondo State, Nigeria, labeled appropriately and taken to the laboratory for analyses. The carrots were divided into 2 groups (A and B). Group A was further divided into subgroups and kept in refrigerator (4 ± 2 <sup>o</sup>C) and colanders (30 ± 2 <sup>o</sup>C) to study the fungi responsible for its spoilage, group B was used to determine the fungi present on carrot. The fungi present on the surface of spoilt carrots and fresh carrots were isolated by scraping the surface of the carrot with sterile knife on sterile alluminium foil. One gram of the scrapped carrot surface was weighed aseptically and diluted serially to dilution 10<sup>-3</sup>. Saboraud agar (SA) and potato dextrose agar (PDA) were used for the culturing, and incubated at 28 <sup>o</sup>C for 72 hours. The morphology of the pure fungus on plate was observed<strong> </strong>for its shape, colour (front and reverse) under the microscope using x10 and x40 objectives. <em>Sacharomyces cerevisiae</em>,<em> Aspergillus nidulans</em>,<em> Pleurothecium recurvatum </em>and<em> Rhizopus nigricans</em> were the fungi isolated from the surface of fresh carrots while (<em>Sclerotini sclerotiorum</em>,<em> Penicillium natatum</em>,<em> Aspergillus niger </em>and<em> Rhizopus nigricans)<strong> </strong></em>were isolated from spoilt carrot. Some of the fungi found on the fresh carrots are pathogenic, fungal species like <em>A. niger</em> could produce toxic metabolites in the affected sites, constituting a potential health hazard for humans, <em>Sclerotini sclerotiorum</em> causes devastating losses to farmers. It is therefore important to look for means of inhibiting the growth of these fungi on carrots to prevent food borne diseases and loss of farm produce. </span></p> |
| title | Fungal Pathogens Responsible for Diseases in Carrot (Daucus Carota l.) Production in Ondo State Nigeria |
| topic | Food-borne diseases food spoilage microflora carrots fungi |
| url | https://doi.org/10.5281/zenodo.18172770 |