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2026
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| Online Access: | https://doi.org/10.5281/zenodo.18181601 |
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| author | Nandani Awadhiya and Arti Saxena |
| author_facet | Nandani Awadhiya and Arti Saxena |
| contents | <div> <p><span lang="EN-US">Anemia is a global public health problem affecting both developing and developed countries with major consequences for human health as well as social and economic development. This problem is the result of a wide variety of causes that can be isolated, but\ more often coexist. Iron-Deficiency Anemia (IDA) is the condition in which the hemoglobin concentration is abnormally low due to iron deficiency, which occurs when there is a long period of negative balance between the amount of biologically available iron and the need for this trace element. On the other hand, iron deficiency or iron depletion is characterized by the decreased iron stores, but the amount of functional iron is not affected. Thus, even though both are related to the same pathological condition, they are characterized by different degrees of deficiency. <span> </span>In our findings, Anaemia patients had markedly reduced levels of weight of men (P=0.0026**) and women (P= P<0.0001***) thus BMI of Women (P=0.0042**) and Men (P=0.0080**) was also significantly different between anaemia patient and healthy tribal population. PCR amplification with specific primers gave 473-bp product which was digested with <em>StuI</em><span> </span>enzyme (New England Biolabs, Boverly, MA) for 16 h at 37˚C. The wild-type genotype (CC) was not digested and gives single 249 bp intact band, whereas the mutated homozygous genotype (TT) was given one band of 125bp <span>(after enzymatic digestion of T allele, gel picture showing single band because of approx same molecular weighted product generated). significant level of change has been seen in overall distribution of </span>TMPRSS6<span> genotypes in Healthy control group as compared to disease group although Healthy control group showed little increase in ‘TT’ genotype as compared to Patients of Anaemia<span> </span>(</span>69.44<span>% <em>vs </em></span>56.25<span>%). Similarly, mutant type ‘CC’ genotype was present in low frequency in<span> </span>Anaemia<span> </span>patients group </span>3.75<span>% and also in control group </span>4.44<span>% (<strong><em><span>χ<sup>2 </sup></span></em></strong><span>= </span></span>0.0243*), P= 7.436)<span>. ‘<em>TT’ </em>genotype is higher in control group and may be protective in our population and statistically significantly different between both groups.</span></span></p> </div> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_18181601 |
| institution | Zenodo |
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| publishDate | 2026 |
| publisher | Zenodo |
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| spellingShingle | Genetic Polymorphism of TMPRSS6 (RS855791) Gene and Its Association with Anaemia in Tribal Population of Madhya Pradesh. Nandani Awadhiya and Arti Saxena <div> <p><span lang="EN-US">Anemia is a global public health problem affecting both developing and developed countries with major consequences for human health as well as social and economic development. This problem is the result of a wide variety of causes that can be isolated, but\ more often coexist. Iron-Deficiency Anemia (IDA) is the condition in which the hemoglobin concentration is abnormally low due to iron deficiency, which occurs when there is a long period of negative balance between the amount of biologically available iron and the need for this trace element. On the other hand, iron deficiency or iron depletion is characterized by the decreased iron stores, but the amount of functional iron is not affected. Thus, even though both are related to the same pathological condition, they are characterized by different degrees of deficiency. <span> </span>In our findings, Anaemia patients had markedly reduced levels of weight of men (P=0.0026**) and women (P= P<0.0001***) thus BMI of Women (P=0.0042**) and Men (P=0.0080**) was also significantly different between anaemia patient and healthy tribal population. PCR amplification with specific primers gave 473-bp product which was digested with <em>StuI</em><span> </span>enzyme (New England Biolabs, Boverly, MA) for 16 h at 37˚C. The wild-type genotype (CC) was not digested and gives single 249 bp intact band, whereas the mutated homozygous genotype (TT) was given one band of 125bp <span>(after enzymatic digestion of T allele, gel picture showing single band because of approx same molecular weighted product generated). significant level of change has been seen in overall distribution of </span>TMPRSS6<span> genotypes in Healthy control group as compared to disease group although Healthy control group showed little increase in ‘TT’ genotype as compared to Patients of Anaemia<span> </span>(</span>69.44<span>% <em>vs </em></span>56.25<span>%). Similarly, mutant type ‘CC’ genotype was present in low frequency in<span> </span>Anaemia<span> </span>patients group </span>3.75<span>% and also in control group </span>4.44<span>% (<strong><em><span>χ<sup>2 </sup></span></em></strong><span>= </span></span>0.0243*), P= 7.436)<span>. ‘<em>TT’ </em>genotype is higher in control group and may be protective in our population and statistically significantly different between both groups.</span></span></p> </div> |
| title | Genetic Polymorphism of TMPRSS6 (RS855791) Gene and Its Association with Anaemia in Tribal Population of Madhya Pradesh. |
| url | https://doi.org/10.5281/zenodo.18181601 |