Cell-Type Specific Variation in X-Chromosome Dosage Compensation in Drosophila
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2026
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| author | Pal, Soumitra |
| author_facet | Pal, Soumitra |
| contents | <p>This repository contains the code and data for the following manuscript in review.</p> <p><strong>Cell-Type Specific Variation in X-Chromosome Dosage Compensation in Drosophila</strong></p> <div> <div> <div> <p>Soumitra Pal, Brian Oliver, Teresa Przytycka</p> </div> </div> </div> <p> </p> <p><strong>ABSTRACT</strong></p> <p>Male <em>Drosophila melanogaster</em> require dosage compensation to equalize X-linked gene expression with autosomal expression. Leveraging the single-nucleus Fly Cell Atlas (FCA) dataset, which includes 388,918 nuclei across diverse tissues, we investigated cell-type-specific patterns of X-chromosome dosage compensation. Our analysis identified a continuum of cell groups based on their X-to-autosome (X/A) expression ratios ranging from anti-compensated to effectively compensated and overcompensated. Anti-compensation was predominantly observed in male reproductive tissues, while overcompensation was prevalent in neural cells. The expression levels of the dosage compensation machinery’s non-coding RNAs, <em>RoX1</em> and <em>RoX2</em>, correlated with compensation levels, but were insufficient to fully explain the observed patterns of compensation. These findings reveal the complexity of dosage compensation and suggest that its regulation by the <em>RoX</em> RNAs is nonlinear, implicating potential alternative mechanisms in certain cell types.</p> <p>Version history:</p> <ul> <li><strong>v0.9.2:</strong> Fixed a missing comma (,) in workflow/scripts/relate_xtoa_rox.R file in source_code.zip.</li> <li><strong>v0.9.1:</strong> Added missing file configs/sex_specific_annotations.csv in the source_code.zip file.</li> <li><strong>v0.9.0:</strong> Added all source code and analysis results.</li> <li><strong>v0.0.1:</strong> An early version of the repository just to get the zenodo links.</li> </ul> <p> </p> |
| format | Recurso digital |
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| publishDate | 2026 |
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| spellingShingle | Cell-Type Specific Variation in X-Chromosome Dosage Compensation in Drosophila Pal, Soumitra <p>This repository contains the code and data for the following manuscript in review.</p> <p><strong>Cell-Type Specific Variation in X-Chromosome Dosage Compensation in Drosophila</strong></p> <div> <div> <div> <p>Soumitra Pal, Brian Oliver, Teresa Przytycka</p> </div> </div> </div> <p> </p> <p><strong>ABSTRACT</strong></p> <p>Male <em>Drosophila melanogaster</em> require dosage compensation to equalize X-linked gene expression with autosomal expression. Leveraging the single-nucleus Fly Cell Atlas (FCA) dataset, which includes 388,918 nuclei across diverse tissues, we investigated cell-type-specific patterns of X-chromosome dosage compensation. Our analysis identified a continuum of cell groups based on their X-to-autosome (X/A) expression ratios ranging from anti-compensated to effectively compensated and overcompensated. Anti-compensation was predominantly observed in male reproductive tissues, while overcompensation was prevalent in neural cells. The expression levels of the dosage compensation machinery’s non-coding RNAs, <em>RoX1</em> and <em>RoX2</em>, correlated with compensation levels, but were insufficient to fully explain the observed patterns of compensation. These findings reveal the complexity of dosage compensation and suggest that its regulation by the <em>RoX</em> RNAs is nonlinear, implicating potential alternative mechanisms in certain cell types.</p> <p>Version history:</p> <ul> <li><strong>v0.9.2:</strong> Fixed a missing comma (,) in workflow/scripts/relate_xtoa_rox.R file in source_code.zip.</li> <li><strong>v0.9.1:</strong> Added missing file configs/sex_specific_annotations.csv in the source_code.zip file.</li> <li><strong>v0.9.0:</strong> Added all source code and analysis results.</li> <li><strong>v0.0.1:</strong> An early version of the repository just to get the zenodo links.</li> </ul> <p> </p> |
| title | Cell-Type Specific Variation in X-Chromosome Dosage Compensation in Drosophila |
| url | https://doi.org/10.5281/zenodo.18235042 |