Preparation of Uniform Rich Cholesterol Unilamellar Nanovesicles Using CO2-Expanded Solvents

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Main Author: Cano-Sarabia, Mary
Format: Recurso digital
Language:English
Published: Zenodo 2026
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author Cano-Sarabia, Mary
author_facet Cano-Sarabia, Mary
contents <p>Nanoscopic and uniform unilamellar vesicles, rich in cholesterol, have been prepared by a new procedure, named “DELOS-SUSP”, which is based on the depressurization of a cholesterol solution in CO<sub>2</sub>-expanded acetone into an aqueous solution containing a surfactant. The CO<sub>2</sub> is used here as a cosolvent medium, allowing the straightforward preparation of vesicular systems with controlled size distribution, uniform shape, and stability unachievable by conventional mixing technologies. The resulting nanoscopic vesicular systems dispersed in water were characterized using dynamic light scattering, ζ potential, turbidity, and cryogenic transmission electron microscopy. The influence of operational parameters of this new methodology on the physicochemical characteristics of the vesicular systems is also reported.</p>
format Recurso digital
id zenodo_https___doi_org_10_5281_zenodo_18356827
institution Zenodo
language eng
publishDate 2026
publisher Zenodo
record_format zenodo
spellingShingle Preparation of Uniform Rich Cholesterol Unilamellar Nanovesicles Using CO2-Expanded Solvents
Cano-Sarabia, Mary
<p>Nanoscopic and uniform unilamellar vesicles, rich in cholesterol, have been prepared by a new procedure, named “DELOS-SUSP”, which is based on the depressurization of a cholesterol solution in CO<sub>2</sub>-expanded acetone into an aqueous solution containing a surfactant. The CO<sub>2</sub> is used here as a cosolvent medium, allowing the straightforward preparation of vesicular systems with controlled size distribution, uniform shape, and stability unachievable by conventional mixing technologies. The resulting nanoscopic vesicular systems dispersed in water were characterized using dynamic light scattering, ζ potential, turbidity, and cryogenic transmission electron microscopy. The influence of operational parameters of this new methodology on the physicochemical characteristics of the vesicular systems is also reported.</p>
title Preparation of Uniform Rich Cholesterol Unilamellar Nanovesicles Using CO2-Expanded Solvents
url https://doi.org/10.5281/zenodo.18356827