Targeted Disruption of Purine Biosynthesis Potently Inhibits Vaccinia Virus Replication In Vitro: A Mechanistic Study on the Antiviral Efficacy of 6-Mercaptopurine
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| Natura: | Recurso digital |
| Lingua: | inglese |
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Zenodo
2022
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| _version_ | 1866901826210627584 |
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| author | Richardson, Alexander L |
| author_facet | Richardson, Alexander L |
| contents | <p>This study provides a detailed investigation into the antiviral potential of 6-mercaptopurine (6-MP) against vaccinia virus <em>in vitro</em>. It demonstrates a potent, dose-dependent inhibition of viral replication, with near-complete suppression achieved at concentrations of 1.0–1.5 µg/mL, while host cell viability remains high. Mechanistic analysis reveals that 6-MP disrupts the viral life cycle by depleting purine nucleotide pools, leading to severe impairment of both viral DNA synthesis and late protein production. The findings establish a clear therapeutic window and confirm the vulnerability of large DNA viruses to host-directed metabolic inhibition, supporting the rationale for exploring purine synthesis inhibitors as broad-spectrum antivirals.</p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_18375903 |
| institution | Zenodo |
| language | eng |
| publishDate | 2022 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | Targeted Disruption of Purine Biosynthesis Potently Inhibits Vaccinia Virus Replication In Vitro: A Mechanistic Study on the Antiviral Efficacy of 6-Mercaptopurine Richardson, Alexander L Vaccinia virus, 6-Mercaptopurine, Antiviral, Nucleotide metabolism, Purine synthesis inhibition, Host-directed therapy, Poxvirus <p>This study provides a detailed investigation into the antiviral potential of 6-mercaptopurine (6-MP) against vaccinia virus <em>in vitro</em>. It demonstrates a potent, dose-dependent inhibition of viral replication, with near-complete suppression achieved at concentrations of 1.0–1.5 µg/mL, while host cell viability remains high. Mechanistic analysis reveals that 6-MP disrupts the viral life cycle by depleting purine nucleotide pools, leading to severe impairment of both viral DNA synthesis and late protein production. The findings establish a clear therapeutic window and confirm the vulnerability of large DNA viruses to host-directed metabolic inhibition, supporting the rationale for exploring purine synthesis inhibitors as broad-spectrum antivirals.</p> |
| title | Targeted Disruption of Purine Biosynthesis Potently Inhibits Vaccinia Virus Replication In Vitro: A Mechanistic Study on the Antiviral Efficacy of 6-Mercaptopurine |
| topic | Vaccinia virus, 6-Mercaptopurine, Antiviral, Nucleotide metabolism, Purine synthesis inhibition, Host-directed therapy, Poxvirus |
| url | https://doi.org/10.5281/zenodo.18375903 |