A COMPARATIVE PHYTOCHEMICAL PROFILING AND IN VITRO ANTIOXIDANT ASSESSMENT OF ORGANICALLY VERSUS CONVENTIONALLY GROWN CABBAGE (BRASSICA OLERACEA)
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| Language: | English |
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Zenodo
2026
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| _version_ | 1866901762802188288 |
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| author | NOUR ALALLAN Dr. LOAI ALALLAN Dr. NASSER THALLAJ |
| author_facet | NOUR ALALLAN Dr. LOAI ALALLAN Dr. NASSER THALLAJ |
| contents | <p><strong><span lang="EN-US">Abstract</span></strong></p> <p><span lang="EN-US">This study presents a comparative analysis of the phytochemical composition and</span><span><span lang="EN-US"> </span></span><em><span lang="EN-US">in vitro</span></em><span lang="EN-US">antioxidant activity of organically and conventionally (termed 'inorganic') cultivated cabbage (<em><span>Brassica oleracea</span></em>) leaves. A multi-methodological approach was employed, encompassing ultrasound-assisted extraction for targeted phenolic recovery, Soxhlet extraction for broad-spectrum analysis, and validated spectrophotometric assays. High-performance liquid chromatography (HPLC) quantification revealed that organic cabbage accumulated significantly higher concentrations of chlorogenic acid (33.86 vs. 14.82 µg/ml), kaempferol (19.87 vs. 12.47 µg/ml), caffeic acid (9.85 vs. 3.11 µg/ml), and quercetin (7.28 vs. 4.87 µg/ml). Gas chromatography-mass spectrometry (GC-MS) profiling further demonstrated distinct compositional differences in volatile and semi-volatile organic constituents between cultivation types. Consistent with these findings, organic extracts exhibited markedly elevated total phenolic (77.12 vs. 53.23 ppm GAE) and total flavonoid (49.43 vs. 32.48 ppm RE) contents. The enhanced phytochemical profile directly correlated with superior antioxidant efficacy, as evidenced by a significantly higher DPPH radical scavenging activity (83% vs. 71%). These results robustly demonstrate that organic cultivation practices significantly enhance the concentration of key bioactive phenolics and the resultant</span><span><span lang="EN-US"> </span></span><em><span lang="EN-US">in vitro</span></em><span><span lang="EN-US"> </span></span><span lang="EN-US">antioxidant capacity of cabbage, underscoring the impact of agricultural methodology on the nutraceutical quality of food crops.</span></p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_18382868 |
| institution | Zenodo |
| language | eng |
| publishDate | 2026 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | A COMPARATIVE PHYTOCHEMICAL PROFILING AND IN VITRO ANTIOXIDANT ASSESSMENT OF ORGANICALLY VERSUS CONVENTIONALLY GROWN CABBAGE (BRASSICA OLERACEA) NOUR ALALLAN Dr. LOAI ALALLAN Dr. NASSER THALLAJ Brassica Oleracea; Organic Cultivation; Phenolic Compounds; HPLC; GC-MS; Antioxidant Activity; DPPH Assay; Phytochemical Profile. <p><strong><span lang="EN-US">Abstract</span></strong></p> <p><span lang="EN-US">This study presents a comparative analysis of the phytochemical composition and</span><span><span lang="EN-US"> </span></span><em><span lang="EN-US">in vitro</span></em><span lang="EN-US">antioxidant activity of organically and conventionally (termed 'inorganic') cultivated cabbage (<em><span>Brassica oleracea</span></em>) leaves. A multi-methodological approach was employed, encompassing ultrasound-assisted extraction for targeted phenolic recovery, Soxhlet extraction for broad-spectrum analysis, and validated spectrophotometric assays. High-performance liquid chromatography (HPLC) quantification revealed that organic cabbage accumulated significantly higher concentrations of chlorogenic acid (33.86 vs. 14.82 µg/ml), kaempferol (19.87 vs. 12.47 µg/ml), caffeic acid (9.85 vs. 3.11 µg/ml), and quercetin (7.28 vs. 4.87 µg/ml). Gas chromatography-mass spectrometry (GC-MS) profiling further demonstrated distinct compositional differences in volatile and semi-volatile organic constituents between cultivation types. Consistent with these findings, organic extracts exhibited markedly elevated total phenolic (77.12 vs. 53.23 ppm GAE) and total flavonoid (49.43 vs. 32.48 ppm RE) contents. The enhanced phytochemical profile directly correlated with superior antioxidant efficacy, as evidenced by a significantly higher DPPH radical scavenging activity (83% vs. 71%). These results robustly demonstrate that organic cultivation practices significantly enhance the concentration of key bioactive phenolics and the resultant</span><span><span lang="EN-US"> </span></span><em><span lang="EN-US">in vitro</span></em><span><span lang="EN-US"> </span></span><span lang="EN-US">antioxidant capacity of cabbage, underscoring the impact of agricultural methodology on the nutraceutical quality of food crops.</span></p> |
| title | A COMPARATIVE PHYTOCHEMICAL PROFILING AND IN VITRO ANTIOXIDANT ASSESSMENT OF ORGANICALLY VERSUS CONVENTIONALLY GROWN CABBAGE (BRASSICA OLERACEA) |
| topic | Brassica Oleracea; Organic Cultivation; Phenolic Compounds; HPLC; GC-MS; Antioxidant Activity; DPPH Assay; Phytochemical Profile. |
| url | https://doi.org/10.5281/zenodo.18382868 |