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2024
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| Online Access: | https://doi.org/10.5281/zenodo.18387313 |
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| author | Balleste, Elisenda Migliorato, Laura Monràs-Riera, Pere Gomez-Herrmann, Pablo Garcia-Aljaro, Cristina |
| author_facet | Balleste, Elisenda Migliorato, Laura Monràs-Riera, Pere Gomez-Herrmann, Pablo Garcia-Aljaro, Cristina |
| contents | In recent years, we conducted several experiments to study the colonization of plastic pellets by aquatic bacteria using microcosm approaches. This study synthesizes the results of various experiments to describe factors affecting colonization. We also assess different techniques to evaluate colonization and calculate microorganism density. Microcosms were set up in oxygenated aquaria of 20-25 L and incubated for 4-6 weeks. The experiments included: i) seawater or freshwater to compare the different type of waters but also the bacterial communities, ii) commercially purchased polymers: polyethylene, polypropylene, polystyrene, and quartz, and environmental plastic pellets collected from a polluted beach, iii) different climatic conditions: Mediterranean and Antarctic, iv) replicates and pseudoreplicates to assess stochastic factors affecting bacterial colonization. We used various techniques to monitor the colonization process, quantifying bacterial density via culture, 16S rRNA gene quantification by qPCR, flow cytometry, and epifluorescence microscopy with DAPI staining. Biofilm structure was evaluated using scanning electron microscopy and high-throughput sequencing of the V4 region of the 16S rRNA gene. Our data suggest that bacteria from different waters rapidly colonize biofilms ( Also see: https://micro2024.sciencesconf.org/559436/document |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_18387313 |
| institution | Zenodo |
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| publishDate | 2024 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | Comparative analysis of factors and methodologies for studying bacterial colonization on plastic pellets using microcosm approaches Balleste, Elisenda Migliorato, Laura Monràs-Riera, Pere Gomez-Herrmann, Pablo Garcia-Aljaro, Cristina colonisation microcosm plastic pellets plastisphere In recent years, we conducted several experiments to study the colonization of plastic pellets by aquatic bacteria using microcosm approaches. This study synthesizes the results of various experiments to describe factors affecting colonization. We also assess different techniques to evaluate colonization and calculate microorganism density. Microcosms were set up in oxygenated aquaria of 20-25 L and incubated for 4-6 weeks. The experiments included: i) seawater or freshwater to compare the different type of waters but also the bacterial communities, ii) commercially purchased polymers: polyethylene, polypropylene, polystyrene, and quartz, and environmental plastic pellets collected from a polluted beach, iii) different climatic conditions: Mediterranean and Antarctic, iv) replicates and pseudoreplicates to assess stochastic factors affecting bacterial colonization. We used various techniques to monitor the colonization process, quantifying bacterial density via culture, 16S rRNA gene quantification by qPCR, flow cytometry, and epifluorescence microscopy with DAPI staining. Biofilm structure was evaluated using scanning electron microscopy and high-throughput sequencing of the V4 region of the 16S rRNA gene. Our data suggest that bacteria from different waters rapidly colonize biofilms ( Also see: https://micro2024.sciencesconf.org/559436/document |
| title | Comparative analysis of factors and methodologies for studying bacterial colonization on plastic pellets using microcosm approaches |
| topic | colonisation microcosm plastic pellets plastisphere |
| url | https://doi.org/10.5281/zenodo.18387313 |