Membrane protein solubilization and structure determination using de novo-designed amphipathic proteins

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Main Authors: Mihaljević, Ljubica, Kim, David E., Bandawane, Pooja D., Eisenach, Helen E., Borst, Andrew J., Courbet, Alexis, Weidle, Connor, Carr, Kenneth D., Bettin, Everton, Liu, Qiushi, Trejos, Aldo T., Majumder, Sagardip, Kokane, Surabhi, Stevens, Alexander, Muratspahić, Edin, Schlichthaerle, Thomas, Exposit, Marc, Li, Xinting, Lamb, Mila, Murray, Analisa Nicole Azcárraga, Ravichandran, Rashmi, Williams, Elizabeth C., Shuyuan, Hu, Stuart, Lynda, Grillová, Linda, Thomson, Nicholas R., Landreh, Michael, Chang, Pengxiang, Giacani, Lorenzo, Caimano, Melissa J., Hawley, Kelly L., King, Neil P., Baker, David
Format: Recurso digital
Published: Zenodo 2026
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author Mihaljević, Ljubica
Kim, David E.
Bandawane, Pooja D.
Eisenach, Helen E.
Borst, Andrew J.
Courbet, Alexis
Weidle, Connor
Carr, Kenneth D.
Bettin, Everton
Liu, Qiushi
Trejos, Aldo T.
Majumder, Sagardip
Kokane, Surabhi
Stevens, Alexander
Muratspahić, Edin
Schlichthaerle, Thomas
Exposit, Marc
Li, Xinting
Lamb, Mila
Murray, Analisa Nicole Azcárraga
Ravichandran, Rashmi
Williams, Elizabeth C.
Shuyuan, Hu
Stuart, Lynda
Grillová, Linda
Thomson, Nicholas R.
Landreh, Michael
Chang, Pengxiang
Giacani, Lorenzo
Caimano, Melissa J.
Hawley, Kelly L.
King, Neil P.
Baker, David
author_facet Mihaljević, Ljubica
Kim, David E.
Bandawane, Pooja D.
Eisenach, Helen E.
Borst, Andrew J.
Courbet, Alexis
Weidle, Connor
Carr, Kenneth D.
Bettin, Everton
Liu, Qiushi
Trejos, Aldo T.
Majumder, Sagardip
Kokane, Surabhi
Stevens, Alexander
Muratspahić, Edin
Schlichthaerle, Thomas
Exposit, Marc
Li, Xinting
Lamb, Mila
Murray, Analisa Nicole Azcárraga
Ravichandran, Rashmi
Williams, Elizabeth C.
Shuyuan, Hu
Stuart, Lynda
Grillová, Linda
Thomson, Nicholas R.
Landreh, Michael
Chang, Pengxiang
Giacani, Lorenzo
Caimano, Melissa J.
Hawley, Kelly L.
King, Neil P.
Baker, David
contents <p>WRAPs (Water-soluble RFdiffused Amphipathic Proteins) are genetically encoded <em>de novo</em> proteins that surround the lipid-interacting hydrophobic surfaces of transmembrane proteins, rendering them thermostable and water-soluble without the need for detergents. This repo includes scripts and inputs to generate WRAPs as described in https://www.biorxiv.org/content/10.1101/2025.02.04.636539v1.</p>
format Recurso digital
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institution Zenodo
language
publishDate 2026
publisher Zenodo
record_format zenodo
spellingShingle Membrane protein solubilization and structure determination using de novo-designed amphipathic proteins
Mihaljević, Ljubica
Kim, David E.
Bandawane, Pooja D.
Eisenach, Helen E.
Borst, Andrew J.
Courbet, Alexis
Weidle, Connor
Carr, Kenneth D.
Bettin, Everton
Liu, Qiushi
Trejos, Aldo T.
Majumder, Sagardip
Kokane, Surabhi
Stevens, Alexander
Muratspahić, Edin
Schlichthaerle, Thomas
Exposit, Marc
Li, Xinting
Lamb, Mila
Murray, Analisa Nicole Azcárraga
Ravichandran, Rashmi
Williams, Elizabeth C.
Shuyuan, Hu
Stuart, Lynda
Grillová, Linda
Thomson, Nicholas R.
Landreh, Michael
Chang, Pengxiang
Giacani, Lorenzo
Caimano, Melissa J.
Hawley, Kelly L.
King, Neil P.
Baker, David
<p>WRAPs (Water-soluble RFdiffused Amphipathic Proteins) are genetically encoded <em>de novo</em> proteins that surround the lipid-interacting hydrophobic surfaces of transmembrane proteins, rendering them thermostable and water-soluble without the need for detergents. This repo includes scripts and inputs to generate WRAPs as described in https://www.biorxiv.org/content/10.1101/2025.02.04.636539v1.</p>
title Membrane protein solubilization and structure determination using de novo-designed amphipathic proteins
url https://doi.org/10.5281/zenodo.18441683