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| Main Author: | |
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| Format: | Recurso digital |
| Language: | English |
| Published: |
Zenodo
2026
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| Online Access: | https://doi.org/10.5281/zenodo.18480497 |
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Table of Contents:
- <p><strong><span>Background:</span></strong><span> Liver cancer remains a major clinical challenge due to its aggressive progression and resistance to conventional therapies. The JAK-STAT signaling pathway and PD-1/PD-L1 immune checkpoint axis are key contributors to tumor growth and immune evasion. <strong>Objective:</strong> This study aimed to evaluate the combined therapeutic effect of Selenium Cysteine (SeCys) and 5-Fluorouracil (5-FU) on tumor growth inhibition, immune modulation, and signaling pathway regulation in a preclinical liver cancer model. <strong>Methods:</strong> We conducted an in vivo study with 250 subjects assigned to control, SeCys, 5-FU, or combination treatment groups. Tumor volumes, survival times, JAK-STAT pathway activation (pJAK2, pSTAT3), PD-L1 expression, and immune cell infiltration (CD8⁺ T-cells) were measured. Statistical analyses included ANOVA, Kruskal–Wallis tests, t-tests, Mann–Whitney U tests, and Pearson correlation. <strong>Results:</strong> Combination therapy significantly inhibited tumor growth (mean TGI 50.82%, p < 0.0001), reduced pSTAT3 levels (χ² = 88.456, p < 0.0001), and increased PD-L1 mRNA expression (t = 8.399, p < 0.0001) compared to controls. </span></p>