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| Format: | Recurso digital |
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Zenodo
2026
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| Online Access: | https://doi.org/10.5281/zenodo.18610350 |
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Table of Contents:
- <p>The current research compares a multifunctional hyaluronic acid (HA)-based biopolymer sheet loaded with <br>hydroxyapatite and melatonin (HHIM) to a plain HA biopolymer sheet for possible bone regeneration usage. <br>Hemocompatibility testing showed that both HA and HHIM scaffolds resulted in very low hemolysis at all concentrations <br>tested; thus, they are compatible with blood. Antibacterial evaluation demonstrated that the HA scaffold and HHIM <br>biopolymer sheet had almost no antimicrobial effects against Streptococcus mutans, Staphylococcus aureus, Pseudomonas <br>aeruginosa and Klebsiella pneumoniae. The antioxidant capacity measured by the DPPH assay revealed a notably higher <br>radical scavenging activity of HHIM with respect to HA, which was concentration-dependent and almost reached the <br>activity of ascorbic acid at the highest concentration. Likewise, the HHIM scaffold has shown higher anti-inflammatory <br>activity, with inhibition values similar to that of diclofenac sodium, while HA had only minor effects. In vitro <br>cytocompatibility tests confirmed that both scaffolds were conducive to cell viability, however, HHIM resulted in better <br>cell adhesion, proliferation, and spreading at 24 and 48 hours. The enhanced biological performance of HHIM makes it a <br>promising candidate as a bioactive scaffold for bone regeneration and maxillofacial tissue engineering. </p>