Integrated Salivary Proteomic and Metabolomic Analyses Reveal Molecular Characterization and Novel Biomarker Panels of Chronic Obstructive Pulmonary Disease

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Main Authors: Xiong, Hangming, Wang, Ping
Format: Recurso digital
Published: Zenodo 2026
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author Xiong, Hangming
Wang, Ping
author_facet Xiong, Hangming
Wang, Ping
contents <h1><span lang="EN-US">Abstract</span></h1> <p><span lang="EN-US">Chronic obstructive pulmonary disease (COPD) is a respiratory disorder characterized by chronic inflammation, oxidative stress, and metabolic dysregulation. The lack of convenient and easily-accessible non-invasive diagnostic approaches remains a major clinical challenge. This study applied an integrated saliva-based proteomic and untargeted metabolomic strategy to identify potential biomarkers for COPD classification. Comprehensive multi-omics analyses identified 225 differentially abundant proteins and 60 differentially abundant metabolites between patients with COPD and healthy controls, including 24 biologically relevant endogenous metabolites. Functional enrichment analyses revealed pronounced dysregulation of mitochondrial energy metabolism, redox homeostasis, lipid remodeling, and inflammatory-related pathways in COPD. By integrating salivary proteomic and metabolomic biomarkers, a stepwise feature selection combined with LASSO logistic regression was used to construct diagnostic models, yielding an optimized biomarker panel consisting of 11 proteins and 2 endogenous metabolites. This integrated model achieved excellent diagnostic performance, with an area under the ROC curve of 0.96. Collectively, these findings demonstrate that integrated salivary proteomic and metabolomic profiling provides a robust, non-invasive approach for COPD classification and offers a promising foundation for the development of biosensor-based diagnostic platforms and early disease detection.</span></p>
format Recurso digital
id zenodo_https___doi_org_10_5281_zenodo_18768828
institution Zenodo
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publishDate 2026
publisher Zenodo
record_format zenodo
spellingShingle Integrated Salivary Proteomic and Metabolomic Analyses Reveal Molecular Characterization and Novel Biomarker Panels of Chronic Obstructive Pulmonary Disease
Xiong, Hangming
Wang, Ping
<h1><span lang="EN-US">Abstract</span></h1> <p><span lang="EN-US">Chronic obstructive pulmonary disease (COPD) is a respiratory disorder characterized by chronic inflammation, oxidative stress, and metabolic dysregulation. The lack of convenient and easily-accessible non-invasive diagnostic approaches remains a major clinical challenge. This study applied an integrated saliva-based proteomic and untargeted metabolomic strategy to identify potential biomarkers for COPD classification. Comprehensive multi-omics analyses identified 225 differentially abundant proteins and 60 differentially abundant metabolites between patients with COPD and healthy controls, including 24 biologically relevant endogenous metabolites. Functional enrichment analyses revealed pronounced dysregulation of mitochondrial energy metabolism, redox homeostasis, lipid remodeling, and inflammatory-related pathways in COPD. By integrating salivary proteomic and metabolomic biomarkers, a stepwise feature selection combined with LASSO logistic regression was used to construct diagnostic models, yielding an optimized biomarker panel consisting of 11 proteins and 2 endogenous metabolites. This integrated model achieved excellent diagnostic performance, with an area under the ROC curve of 0.96. Collectively, these findings demonstrate that integrated salivary proteomic and metabolomic profiling provides a robust, non-invasive approach for COPD classification and offers a promising foundation for the development of biosensor-based diagnostic platforms and early disease detection.</span></p>
title Integrated Salivary Proteomic and Metabolomic Analyses Reveal Molecular Characterization and Novel Biomarker Panels of Chronic Obstructive Pulmonary Disease
url https://doi.org/10.5281/zenodo.18768828