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Bibliographic Details
Main Authors:	del Puerto Nevado, Laura, Santiago Hernandez, Aránzazu, Sonia Solanes Casado, González, Nieves, Marta Ricole Vila, Corton, Marta, Prieto, Isabel, Mas, Sebastian, Sanz, Ana Belen, aguilera, oscar, Gomez-Guerrero, Carmen, Ayuso, Carmen, Ortiz Arduan, Alberto, Rojo, Federico, Egido, Jesus, J, Garcia-Foncillas, Minguez, Pablo, Alvarez-Llamas, Gloria
Format:	Recurso digital
Language:	English
Published:	Zenodo 2019
Subjects:	colon cancer; diabetes; field of cancerization; mitochondria; proteomics
Online Access:	https://doi.org/10.5281/zenodo.18772534
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Table of Contents:

Type 2 diabetes mellitus (T2DM) has been associated with an increased risk of cancer, including colon cancer (CC). However, we recently reported no influence of T2DM on CC prognosis, suggesting that any effect might be at the early stages of tumor development. We hypothesized that T2DM may create an environment in the healthy tissue, which acts as a carcinogenesis driver in agreement with the field of cancerization concept. Here, we focused on early carcinogenesis by analyzing paired tumor and normal colonic mucosa samples from the same patients. The proteome of CC and paired mucosa was quantitatively analyzed in 28 individuals (12 diabetics and 16 nondiabetics) by mass spectrometry with isobaric labeling. Out of 3076 identified proteins, 425 were differentially expressed at the tumor in diabetics compared with nondiabetics. In the adjacent mucosa, 143 proteins were differentially expressed in diabetics and nondiabetics. An enrichment analysis of this signature pointed to mitochondria, ribosome, and translation. Only six proteins were upregulated by diabetes both in tumor and mucosa, of which five were mitochondrial proteins. Differential expression in diabetic versus nondiabetic mucosa was confirmed for MRPL53, MRPL18, and TIMM8B. Higher levels of MRPL18, TIMM8B, and EIF1A were also found in normal colon epithelial cells exposed to high-glucose conditions. We conclude that T2DM is associated with specific molecular changes in the normal mucosa of CC patients, consistent with field of cancerization in a diabetic environment. The mitochondrial protein signature identifies a potential therapeutic target that could underlie the higher risk of CC in diabetics.

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