Detection of Vancomycin-Resistant Enterococci from Clinical Specimens in a Tertiary Care Hospital in Western Rajasthan: An Observational Study
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2026
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| author | Smita kulshrestha, Dr. Dheeraj Kumar Khandelwal*, Durga prasad, Ritu gupta |
| author_facet | Smita kulshrestha, Dr. Dheeraj Kumar Khandelwal*, Durga prasad, Ritu gupta |
| contents | <p><span>Aims & Background: Antimicrobial resistance (AMR) has become an emerging global health challenge now a day. Enterococci are opportunistic pathogens and are responsible for various types of infections. The growing clinical importance of these organisms is associated with their ability to show resistance to various types of antibiotics. Thus this study was planed to detect the prevalence of Vancomycin Resistant Enterococci (VRE) in different clinical isolates. Materials and Methods: This observational study was carried out on 550Enterococci isolates in Department of Microbiology, Dr S N Medical College, Jodhpur. Specimens<span> </span>were<span> </span>processed<span> </span>according<span> </span>to<span> </span>standard<span> </span>procedures.Inoculation was done on blood agar and MacConkey agar. Additionally, chocolate agar was use for CSF specimens. Identification of isolates wasbased on colony morphology, Gram’s stain appearance and a negative catalase reaction with growth on bile esculin agar. Further subspecies were identified based on their biochemical profiles. Final confirmation of species was done by Vitek 2 system. Kirby Bauer disk diffusion method is used to find out phenotypic resistance patterns among all isolated enterococci</span></p> <p><span>Results:Out of 550 isolates 457 (83.09%) were identified as E. faecalis, and 88 (16.0%) were E. Faecium and 5 were other species.Among the enterococcal isolates, enterococci were more sensitive to antibiotics vancomycin (94.0%) and linezolid (100%).6% of isolates (33 isolates) showed resistance towards vancomycin. The vancomycin-resistant strains of E. Faecalis (n=8)and E. Faecium (n=25)showed significant resistance to all the other antibiotics tested, except for linezolid.Conclusion: Findings of this study indicates a higher rate of vancomycin resistance (6%) in our region.This study shows geographical variations in the isolation and distribution of Enterococcus species, along with notable variation in vancomycin resistance. <span> </span>Clinical significance: Geographical variations should be considered as an adjunct in selecting antibiotic for treatment of </span></p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_18981330 |
| institution | Zenodo |
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| publishDate | 2026 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | Detection of Vancomycin-Resistant Enterococci from Clinical Specimens in a Tertiary Care Hospital in Western Rajasthan: An Observational Study Smita kulshrestha, Dr. Dheeraj Kumar Khandelwal*, Durga prasad, Ritu gupta Enterococci, Vancomycin, antibiotics resistance <p><span>Aims & Background: Antimicrobial resistance (AMR) has become an emerging global health challenge now a day. Enterococci are opportunistic pathogens and are responsible for various types of infections. The growing clinical importance of these organisms is associated with their ability to show resistance to various types of antibiotics. Thus this study was planed to detect the prevalence of Vancomycin Resistant Enterococci (VRE) in different clinical isolates. Materials and Methods: This observational study was carried out on 550Enterococci isolates in Department of Microbiology, Dr S N Medical College, Jodhpur. Specimens<span> </span>were<span> </span>processed<span> </span>according<span> </span>to<span> </span>standard<span> </span>procedures.Inoculation was done on blood agar and MacConkey agar. Additionally, chocolate agar was use for CSF specimens. Identification of isolates wasbased on colony morphology, Gram’s stain appearance and a negative catalase reaction with growth on bile esculin agar. Further subspecies were identified based on their biochemical profiles. Final confirmation of species was done by Vitek 2 system. Kirby Bauer disk diffusion method is used to find out phenotypic resistance patterns among all isolated enterococci</span></p> <p><span>Results:Out of 550 isolates 457 (83.09%) were identified as E. faecalis, and 88 (16.0%) were E. Faecium and 5 were other species.Among the enterococcal isolates, enterococci were more sensitive to antibiotics vancomycin (94.0%) and linezolid (100%).6% of isolates (33 isolates) showed resistance towards vancomycin. The vancomycin-resistant strains of E. Faecalis (n=8)and E. Faecium (n=25)showed significant resistance to all the other antibiotics tested, except for linezolid.Conclusion: Findings of this study indicates a higher rate of vancomycin resistance (6%) in our region.This study shows geographical variations in the isolation and distribution of Enterococcus species, along with notable variation in vancomycin resistance. <span> </span>Clinical significance: Geographical variations should be considered as an adjunct in selecting antibiotic for treatment of </span></p> |
| title | Detection of Vancomycin-Resistant Enterococci from Clinical Specimens in a Tertiary Care Hospital in Western Rajasthan: An Observational Study |
| topic | Enterococci, Vancomycin, antibiotics resistance |
| url | https://doi.org/10.5281/zenodo.18981330 |