| _version_ | 1866902219386781696 |
|---|---|
| author | Talha315 Farhana Jahan |
| author_facet | Talha315 Farhana Jahan |
| contents | <p>This release archives the image-analysis pipeline used in the associated manuscript describing the ex ovo intracardiac neuroblastoma model for evaluating NK–tumor interactions.</p> <p>The repository contains scripts for fluorescence image processing and quantitative analysis, including CellPose-based cell segmentation, Otsu threshold refinement, background correction, automated cell detection, and spatial proximity analysis between NK cells and GFP⁺ neuroblastoma cells using a calibrated 75 µm interaction radius. The workflow was applied to both ex ovo chick embryo imaging datasets and in vitro co-culture assays.</p> <p>The README file includes tables summarizing adjustable segmentation and classification parameters to facilitate reproducibility and adaptation of the pipeline.</p> <p>This archived release ensures reproducibility of the computational workflow described in the protocol.</p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_19029049 |
| institution | Zenodo |
| language | |
| publishDate | 2026 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | F-07-J/ExOvo_Intracardiac_Method_Article: Analysis pipeline for Ex-Ovo NK–tumor interaction study Talha315 Farhana Jahan <p>This release archives the image-analysis pipeline used in the associated manuscript describing the ex ovo intracardiac neuroblastoma model for evaluating NK–tumor interactions.</p> <p>The repository contains scripts for fluorescence image processing and quantitative analysis, including CellPose-based cell segmentation, Otsu threshold refinement, background correction, automated cell detection, and spatial proximity analysis between NK cells and GFP⁺ neuroblastoma cells using a calibrated 75 µm interaction radius. The workflow was applied to both ex ovo chick embryo imaging datasets and in vitro co-culture assays.</p> <p>The README file includes tables summarizing adjustable segmentation and classification parameters to facilitate reproducibility and adaptation of the pipeline.</p> <p>This archived release ensures reproducibility of the computational workflow described in the protocol.</p> |
| title | F-07-J/ExOvo_Intracardiac_Method_Article: Analysis pipeline for Ex-Ovo NK–tumor interaction study |
| url | https://doi.org/10.5281/zenodo.19029049 |