Development and validation of RP-HPLC method for determination of propofol in bulk and pharmaceutical dosage form.
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| Formato: | Recurso digital |
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2026
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| _version_ | 1866901251249143808 |
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| author | Dr. Shweta Singh Dr. Avadhesh Diwakar |
| author_facet | Dr. Shweta Singh Dr. Avadhesh Diwakar |
| contents | <p><span>The aim of this study was to develop a simple, precise, accurate,<br>economic and reproducible RP-HPLC method for determination<br>of propofol in bulk and pharmaceutical dosage form and<br>validate as per ICH guidelines. In this analysis using C</span><span>18<br></span><span>column and its dimensions is 250 x 4.6 mm, 5µm with methanol<br>and water for injection as mobile phase in the ratio of 84:16 at<br>flow rate of 0.8 ml/min and detection was carried out at 275 nm<br>by using UV visible detector. The injection volume is 20 µl and<br>retention time was found to be 7min with 18 min as run time. In<br>HPLC method linearity was found in 10-50 µg/ml with<br>correlation coefficient 0.998. The percentage recovery was<br>found to be 99.1 to 100.3 %. The proposed method gave good</span> </p> <p><span>separation for propofol and method was validated for accuracy,<br>precision, linearity, limit of detection, limit of quantitation,<br>robustness and ruggedness. The result of study showed that the<br>proposed method RP-HPLC is suitable for routine analysis of<br>itraconazole in bulk and pharmaceutical dosage form.</span> </p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_19149438 |
| institution | Zenodo |
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| publishDate | 2026 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | Development and validation of RP-HPLC method for determination of propofol in bulk and pharmaceutical dosage form. Dr. Shweta Singh Dr. Avadhesh Diwakar <p><span>The aim of this study was to develop a simple, precise, accurate,<br>economic and reproducible RP-HPLC method for determination<br>of propofol in bulk and pharmaceutical dosage form and<br>validate as per ICH guidelines. In this analysis using C</span><span>18<br></span><span>column and its dimensions is 250 x 4.6 mm, 5µm with methanol<br>and water for injection as mobile phase in the ratio of 84:16 at<br>flow rate of 0.8 ml/min and detection was carried out at 275 nm<br>by using UV visible detector. The injection volume is 20 µl and<br>retention time was found to be 7min with 18 min as run time. In<br>HPLC method linearity was found in 10-50 µg/ml with<br>correlation coefficient 0.998. The percentage recovery was<br>found to be 99.1 to 100.3 %. The proposed method gave good</span> </p> <p><span>separation for propofol and method was validated for accuracy,<br>precision, linearity, limit of detection, limit of quantitation,<br>robustness and ruggedness. The result of study showed that the<br>proposed method RP-HPLC is suitable for routine analysis of<br>itraconazole in bulk and pharmaceutical dosage form.</span> </p> |
| title | Development and validation of RP-HPLC method for determination of propofol in bulk and pharmaceutical dosage form. |
| url | https://doi.org/10.5281/zenodo.19149438 |