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| Format: | Recurso digital |
| Sprache: | Englisch |
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2026
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| Online-Zugang: | https://doi.org/10.5281/zenodo.19331042 |
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| author | Rikhsiboev, Oybek Rasakhadjayev, Bahramjan |
| author_facet | Rikhsiboev, Oybek Rasakhadjayev, Bahramjan |
| contents | <p><span lang="EN-US">Anaerobic digestion (AD) is a cornerstone technology for sustainable waste management and renewable energy production, yet hydrolysis remains the rate-limiting step in the conversion of complex organic matter to biogas (Appels et al., 2011). Here we isolate and characterize <em>Clostridium hydrolyticum</em>sp. nov., a novel bacterial strain with exceptional extracellular hydrolytic capabilities. Through comprehensive genomic, transcriptomic, and metabolomic analyses, we demonstrate that this strain produces a diverse array of carbohydrate-active enzymes (CAZymes), including novel cellulases, hemicellulases, and proteases with activity across broad pH and temperature ranges. When introduced to mesophilic (35-37 degrees C), thermophilic (50-55 degrees C), and hyperthermophilic (70 degrees C) anaerobic digesters treating agricultural waste, food waste, and sewage sludge, the strain enhanced methane yields by 23-41% and reduced hydraulic retention time requirements by 15-28%. Mechanistic studies reveal that <em>C. hydrolyticum</em>establishes synergistic relationships with methanogenic archaea through metabolic cross-feeding and biofilm formation (Westerholm et al., 2016). These findings establish <em>C. hydrolyticum</em>as a powerful bioaugmentation agent for improving AD efficiency across diverse operational conditions, with significant implications for the biogas industry and circular economy initiatives.</span></p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_19331042 |
| institution | Zenodo |
| language | eng |
| publishDate | 2026 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | A Novel Hydrolytic Bacterial Strain for Improved Anaerobic Digestion: Effects Across Different Digester Types Rikhsiboev, Oybek Rasakhadjayev, Bahramjan (4-(m-Chlorophenylcarbamoyloxy)-2-butynyl)trimethylammonium Chloride/analysis Biogas Bacteria, Anaerobic <p><span lang="EN-US">Anaerobic digestion (AD) is a cornerstone technology for sustainable waste management and renewable energy production, yet hydrolysis remains the rate-limiting step in the conversion of complex organic matter to biogas (Appels et al., 2011). Here we isolate and characterize <em>Clostridium hydrolyticum</em>sp. nov., a novel bacterial strain with exceptional extracellular hydrolytic capabilities. Through comprehensive genomic, transcriptomic, and metabolomic analyses, we demonstrate that this strain produces a diverse array of carbohydrate-active enzymes (CAZymes), including novel cellulases, hemicellulases, and proteases with activity across broad pH and temperature ranges. When introduced to mesophilic (35-37 degrees C), thermophilic (50-55 degrees C), and hyperthermophilic (70 degrees C) anaerobic digesters treating agricultural waste, food waste, and sewage sludge, the strain enhanced methane yields by 23-41% and reduced hydraulic retention time requirements by 15-28%. Mechanistic studies reveal that <em>C. hydrolyticum</em>establishes synergistic relationships with methanogenic archaea through metabolic cross-feeding and biofilm formation (Westerholm et al., 2016). These findings establish <em>C. hydrolyticum</em>as a powerful bioaugmentation agent for improving AD efficiency across diverse operational conditions, with significant implications for the biogas industry and circular economy initiatives.</span></p> |
| title | A Novel Hydrolytic Bacterial Strain for Improved Anaerobic Digestion: Effects Across Different Digester Types |
| topic | (4-(m-Chlorophenylcarbamoyloxy)-2-butynyl)trimethylammonium Chloride/analysis Biogas Bacteria, Anaerobic |
| url | https://doi.org/10.5281/zenodo.19331042 |