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| Main Authors: | , |
|---|---|
| Format: | Recurso digital |
| Language: | English |
| Published: |
Zenodo
2026
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| Subjects: | |
| Online Access: | https://doi.org/10.5281/zenodo.19331042 |
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Table of Contents:
- <p><span lang="EN-US">Anaerobic digestion (AD) is a cornerstone technology for sustainable waste management and renewable energy production, yet hydrolysis remains the rate-limiting step in the conversion of complex organic matter to biogas (Appels et al., 2011). Here we isolate and characterize <em>Clostridium hydrolyticum</em>sp. nov., a novel bacterial strain with exceptional extracellular hydrolytic capabilities. Through comprehensive genomic, transcriptomic, and metabolomic analyses, we demonstrate that this strain produces a diverse array of carbohydrate-active enzymes (CAZymes), including novel cellulases, hemicellulases, and proteases with activity across broad pH and temperature ranges. When introduced to mesophilic (35-37 degrees C), thermophilic (50-55 degrees C), and hyperthermophilic (70 degrees C) anaerobic digesters treating agricultural waste, food waste, and sewage sludge, the strain enhanced methane yields by 23-41% and reduced hydraulic retention time requirements by 15-28%. Mechanistic studies reveal that <em>C. hydrolyticum</em>establishes synergistic relationships with methanogenic archaea through metabolic cross-feeding and biofilm formation (Westerholm et al., 2016). These findings establish <em>C. hydrolyticum</em>as a powerful bioaugmentation agent for improving AD efficiency across diverse operational conditions, with significant implications for the biogas industry and circular economy initiatives.</span></p>