Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Recurso digital |
| Language: | |
| Published: |
Zenodo
2026
|
| Online Access: | https://doi.org/10.5281/zenodo.19481133 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1866902107644231680 |
|---|---|
| author | BYUN, Younsoo Noh, Hyunjun Rhim, Sung-Han Noh, Jihyun |
| author_facet | BYUN, Younsoo Noh, Hyunjun Rhim, Sung-Han Noh, Jihyun |
| contents | <p><span>Brain slice electrophysiology is a widely used approach for investigating synaptic physiology, network activity, and pharmacological responses. However, the high cost of commercial recording chambers and perfusion systems restricts accessibility in resource-limited laboratories. Here, we describe the design, fabrication, and electrophysiological validation of a fully integrated, open-source field recording platform for acute brain slice electrophysiology. The system comprises three independently assembled modules: a submerged recording chamber, a tissue-positioning stage, and a suction assembly, all fabricated via fused deposition modeling using polylactic acid filaments at a total material cost of approximately 1.23 USD. The platform was validated using acute hippocampal slices from adult male C57BL/6N mice. Field excitatory postsynaptic potential recordings from the CA3-CA1 Schaffer collateral pathway demonstrated high-fidelity signals and reproducible stimulus-response relationships, alongside stable 15-minute baselines. Thermal characterization confirmed uniform perfusate distribution and rapid temperature equilibration within the chamber bath. All computer-aided design files and assembly instructions are openly available under a CC BY 4.0 license, enabling straightforward replication by the broader neuroscience community.</span></p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_19481133 |
| institution | Zenodo |
| language | |
| publishDate | 2026 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | Design files for a low-cost 3D-printed open-source platform for acute brain slice electrophysiology BYUN, Younsoo Noh, Hyunjun Rhim, Sung-Han Noh, Jihyun <p><span>Brain slice electrophysiology is a widely used approach for investigating synaptic physiology, network activity, and pharmacological responses. However, the high cost of commercial recording chambers and perfusion systems restricts accessibility in resource-limited laboratories. Here, we describe the design, fabrication, and electrophysiological validation of a fully integrated, open-source field recording platform for acute brain slice electrophysiology. The system comprises three independently assembled modules: a submerged recording chamber, a tissue-positioning stage, and a suction assembly, all fabricated via fused deposition modeling using polylactic acid filaments at a total material cost of approximately 1.23 USD. The platform was validated using acute hippocampal slices from adult male C57BL/6N mice. Field excitatory postsynaptic potential recordings from the CA3-CA1 Schaffer collateral pathway demonstrated high-fidelity signals and reproducible stimulus-response relationships, alongside stable 15-minute baselines. Thermal characterization confirmed uniform perfusate distribution and rapid temperature equilibration within the chamber bath. All computer-aided design files and assembly instructions are openly available under a CC BY 4.0 license, enabling straightforward replication by the broader neuroscience community.</span></p> |
| title | Design files for a low-cost 3D-printed open-source platform for acute brain slice electrophysiology |
| url | https://doi.org/10.5281/zenodo.19481133 |