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| Main Authors: | , , , |
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| Format: | Recurso digital |
| Language: | |
| Published: |
Zenodo
2026
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| Online Access: | https://doi.org/10.5281/zenodo.19481133 |
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Table of Contents:
- <p><span>Brain slice electrophysiology is a widely used approach for investigating synaptic physiology, network activity, and pharmacological responses. However, the high cost of commercial recording chambers and perfusion systems restricts accessibility in resource-limited laboratories. Here, we describe the design, fabrication, and electrophysiological validation of a fully integrated, open-source field recording platform for acute brain slice electrophysiology. The system comprises three independently assembled modules: a submerged recording chamber, a tissue-positioning stage, and a suction assembly, all fabricated via fused deposition modeling using polylactic acid filaments at a total material cost of approximately 1.23 USD. The platform was validated using acute hippocampal slices from adult male C57BL/6N mice. Field excitatory postsynaptic potential recordings from the CA3-CA1 Schaffer collateral pathway demonstrated high-fidelity signals and reproducible stimulus-response relationships, alongside stable 15-minute baselines. Thermal characterization confirmed uniform perfusate distribution and rapid temperature equilibration within the chamber bath. All computer-aided design files and assembly instructions are openly available under a CC BY 4.0 license, enabling straightforward replication by the broader neuroscience community.</span></p>