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Zenodo
2026
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| Accesso online: | https://doi.org/10.5281/zenodo.19600813 |
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| author | Busquets, Oriol Li, Hanqin Syed, Khaja Mohieddin Alvarez-Jerez, Pilar Dunnack, Jesse Lo Bu, Riana Verma, Yogendra Pangilinan, Gabriella R. Martin, Annika Straub, Jannes Du, YuXin Simon, Vivien M. Poser, Steven Bush, Zipporiah Diaz, Jessica Sahagun, Atehsa Gao, Jianpu Hong, Samantha Hernandez, Dena G. Levine, Kristin S. Pochet, Nathalie Booth, Ezgi O. Blanchette, Marco Bateup, Helen S. Rio, Donald C. Blauwendraat, Cornelis Hockemeyer, Dirk Soldner, Frank |
| author_facet | Busquets, Oriol Li, Hanqin Syed, Khaja Mohieddin Alvarez-Jerez, Pilar Dunnack, Jesse Lo Bu, Riana Verma, Yogendra Pangilinan, Gabriella R. Martin, Annika Straub, Jannes Du, YuXin Simon, Vivien M. Poser, Steven Bush, Zipporiah Diaz, Jessica Sahagun, Atehsa Gao, Jianpu Hong, Samantha Hernandez, Dena G. Levine, Kristin S. Pochet, Nathalie Booth, Ezgi O. Blanchette, Marco Bateup, Helen S. Rio, Donald C. Blauwendraat, Cornelis Hockemeyer, Dirk Soldner, Frank |
| contents | <p>This Zenodo deposit contains a publicly available description of the Dataset:</p> <p><strong>Title:</strong> "iSCORE-PD: NGS genotyping and zygosity testing of isogenic hESC clones".</p> <p><strong>Description:</strong> Targeted DNA sequencing data for the iSCORE-PD isogenic hESC clone collection, encompassing three complementary analyses: (1) NGS genotyping of 53 mutant clones across 11 PD-linked genes via amplicon sequencing on Illumina iSeq 100 with CRISPResso2 analysis, (2) zygosity testing of a subset of clones in 6 genes via SNP-PCR (NGS or Sanger) to assess zygosity near CRISPR edit sites, and (3) Sanger sequencing of the wild-type allele in 5 DJ1/PARK7 heterozygous clones.</p> <blockquote> <p>This dataset is made available to researchers via the ASAP CRN Cloud: <a href="https://cloud.parkinsonsroadmap.org">cloud.parkinsonsroadmap.org</a>. Instructions for how to request access can be found in the <a href="https://storage.googleapis.com/asap-public-assets/wayfinding/ASAP-CRN-Cloud-User-Manual.pdf">User Manual</a>.</p> <p>This research was funded by the Aligning Science Across Parkinson's Collaborative Research Network (ASAP CRN), through the Michael J. Fox Foundation for Parkinson's Research (MJFF).</p> <p>This Zenodo deposit was created by the ASAP CRN Cloud staff on behalf of the dataset authors. It provides a citable reference for a CRN Cloud Dataset</p> </blockquote> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_19600813 |
| institution | Zenodo |
| language | |
| publishDate | 2026 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | iSCORE-PD: NGS genotyping and zygosity testing of isogenic hESC clones Busquets, Oriol Li, Hanqin Syed, Khaja Mohieddin Alvarez-Jerez, Pilar Dunnack, Jesse Lo Bu, Riana Verma, Yogendra Pangilinan, Gabriella R. Martin, Annika Straub, Jannes Du, YuXin Simon, Vivien M. Poser, Steven Bush, Zipporiah Diaz, Jessica Sahagun, Atehsa Gao, Jianpu Hong, Samantha Hernandez, Dena G. Levine, Kristin S. Pochet, Nathalie Booth, Ezgi O. Blanchette, Marco Bateup, Helen S. Rio, Donald C. Blauwendraat, Cornelis Hockemeyer, Dirk Soldner, Frank <p>This Zenodo deposit contains a publicly available description of the Dataset:</p> <p><strong>Title:</strong> "iSCORE-PD: NGS genotyping and zygosity testing of isogenic hESC clones".</p> <p><strong>Description:</strong> Targeted DNA sequencing data for the iSCORE-PD isogenic hESC clone collection, encompassing three complementary analyses: (1) NGS genotyping of 53 mutant clones across 11 PD-linked genes via amplicon sequencing on Illumina iSeq 100 with CRISPResso2 analysis, (2) zygosity testing of a subset of clones in 6 genes via SNP-PCR (NGS or Sanger) to assess zygosity near CRISPR edit sites, and (3) Sanger sequencing of the wild-type allele in 5 DJ1/PARK7 heterozygous clones.</p> <blockquote> <p>This dataset is made available to researchers via the ASAP CRN Cloud: <a href="https://cloud.parkinsonsroadmap.org">cloud.parkinsonsroadmap.org</a>. Instructions for how to request access can be found in the <a href="https://storage.googleapis.com/asap-public-assets/wayfinding/ASAP-CRN-Cloud-User-Manual.pdf">User Manual</a>.</p> <p>This research was funded by the Aligning Science Across Parkinson's Collaborative Research Network (ASAP CRN), through the Michael J. Fox Foundation for Parkinson's Research (MJFF).</p> <p>This Zenodo deposit was created by the ASAP CRN Cloud staff on behalf of the dataset authors. It provides a citable reference for a CRN Cloud Dataset</p> </blockquote> |
| title | iSCORE-PD: NGS genotyping and zygosity testing of isogenic hESC clones |
| url | https://doi.org/10.5281/zenodo.19600813 |