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| Format: | Recurso digital |
| Language: | English |
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2026
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| Online Access: | https://doi.org/10.5281/zenodo.19678895 |
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| _version_ | 1866901888532742144 |
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| author | Pham, Thi Loc |
| author_facet | Pham, Thi Loc |
| contents | <p>Large-scale somatic mutation data from COSMIC v97, GenomePaint (St. Jude Cloud), and TARGET/TCGA (N = 1,392 T-ALL samples) were integrated to identify high-frequency hotspot mutations at exons 26–27. Five MHC class I neoepitopes were predicted and stringently filtered using NetMHCpan-4.1, HLAthena, and NetMHCstabpan against a Vietnamese-prevalent HLA allele panel. A multicomponent vaccine construct was assembled incorporating the molecular adjuvant CTB, two universal T-helper epitopes (P2/P30), three MHC class II helper epitopes from NOTCH1 and RHEB, one linear B-cell epitope from NOTCH1, and a clinically validated modified WT1 epitope (CYTWNQMNL). The construct underwent comprehensive evaluation of physicochemical and immunological properties, 3D structure prediction (AlphaFold2/RoseTTAFold2), molecular docking (AutoDock Vina, PatchDock/FireDock), immune simulation (C-ImmSim), and codon optimization (JCat).</p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_19678895 |
| institution | Zenodo |
| language | eng |
| publishDate | 2026 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | PERSONALIZED MULTI-EPITOPE PEPTIDE VACCINE DESIGN TARGETING NOTCH1 MUTATIONS IN T-CELL ACUTE LYMPHOBLASTIC LEUKEMIA (T-ALL): A COMPREHENSIVE IMMUNOINFORMATICS ANALYSIS Pham, Thi Loc <p>Large-scale somatic mutation data from COSMIC v97, GenomePaint (St. Jude Cloud), and TARGET/TCGA (N = 1,392 T-ALL samples) were integrated to identify high-frequency hotspot mutations at exons 26–27. Five MHC class I neoepitopes were predicted and stringently filtered using NetMHCpan-4.1, HLAthena, and NetMHCstabpan against a Vietnamese-prevalent HLA allele panel. A multicomponent vaccine construct was assembled incorporating the molecular adjuvant CTB, two universal T-helper epitopes (P2/P30), three MHC class II helper epitopes from NOTCH1 and RHEB, one linear B-cell epitope from NOTCH1, and a clinically validated modified WT1 epitope (CYTWNQMNL). The construct underwent comprehensive evaluation of physicochemical and immunological properties, 3D structure prediction (AlphaFold2/RoseTTAFold2), molecular docking (AutoDock Vina, PatchDock/FireDock), immune simulation (C-ImmSim), and codon optimization (JCat).</p> |
| title | PERSONALIZED MULTI-EPITOPE PEPTIDE VACCINE DESIGN TARGETING NOTCH1 MUTATIONS IN T-CELL ACUTE LYMPHOBLASTIC LEUKEMIA (T-ALL): A COMPREHENSIVE IMMUNOINFORMATICS ANALYSIS |
| url | https://doi.org/10.5281/zenodo.19678895 |