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Main Authors: Hillary Nepon, Nikita Kalashnikov, Sarah Petrecca, Tassos Dionisopoulos, Peter Davison, Tyler Safran, Joshua Vorstenbosch
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Published: Zenodo 2026
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Online Access:https://doi.org/10.5281/zenodo.20061733
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author Hillary Nepon
Nikita Kalashnikov
Sarah Petrecca
Tassos Dionisopoulos
Peter Davison
Tyler Safran
Joshua Vorstenbosch
author_facet Hillary Nepon
Nikita Kalashnikov
Sarah Petrecca
Tassos Dionisopoulos
Peter Davison
Tyler Safran
Joshua Vorstenbosch
contents PURPOSE: Radiation-induced capsular contracture (RICC) commonly affects breast reconstruction patients leading to patient morbidity. The pathoaetiology of RICC remains poorly understood, including the roles of the surrounding tissue environment and mechanical signaling. This study aimed to characterize the inflammatory, fibrotic and mechanical signaling profile of radiated human breast implant capsule depending on the tissue type in contact with the implant. METHODS: Capsule tissue was collected from 16 radiated and 15 non-radiated control breast reconstruction patients, including capsule adjacent to skin and capsule adjacent to muscle utilizing a within-patient design. Samples were analyzed with histology, immunohistochemistry and q-PCR to measure inflammatory, fibrotic and YAP/TAZ mechanical signaling markers. RESULTS: Radiated capsule displayed significantly abnormal morphology, increased immune and fibroblast populations and YAP/TAZ-positive cells compared to control capsule. Capsule adjacent to radiated skin displayed abnormal collagen deposition, increased pro-inflammatory M1 macrophages, pro-fibrotic CD26+ fibroblasts, YAP/TAZ-positive cells and expression of IL-6, collagen type 1,TGF-β, YAP/TAZ in comparison to capsule that developed adjacent to radiated muscle. CONCLUSION: In this study, we show that the radiated tissue microenvironment significantly alters the inflammatory and fibrotic phenotype of human breast implant capsule. We also identify a novel link between radiation-induced capsular contracture and YAP/TAZ mechanical signaling which is further modified depending on the adjacent tissue type. This knowledge may help improve our understanding of the pathoaetiology of RICC and identifies a potential molecular therapeutic target to improve breast reconstructive outcomes in this high-risk population.© 2026. Plastic Surgery Research Council | All rights reserved |*Source: https://ps-rc.org/meeting/Program/2026/OS01.cgi*
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spellingShingle The Adjacent Tissue Environment And Mechanical Signaling Influence Radiation-induced Capsule Fibrosis
Hillary Nepon
Nikita Kalashnikov
Sarah Petrecca
Tassos Dionisopoulos
Peter Davison
Tyler Safran
Joshua Vorstenbosch
plastic surgery
reconstructive surgery
conference abstract
PSRC 2026
PURPOSE: Radiation-induced capsular contracture (RICC) commonly affects breast reconstruction patients leading to patient morbidity. The pathoaetiology of RICC remains poorly understood, including the roles of the surrounding tissue environment and mechanical signaling. This study aimed to characterize the inflammatory, fibrotic and mechanical signaling profile of radiated human breast implant capsule depending on the tissue type in contact with the implant. METHODS: Capsule tissue was collected from 16 radiated and 15 non-radiated control breast reconstruction patients, including capsule adjacent to skin and capsule adjacent to muscle utilizing a within-patient design. Samples were analyzed with histology, immunohistochemistry and q-PCR to measure inflammatory, fibrotic and YAP/TAZ mechanical signaling markers. RESULTS: Radiated capsule displayed significantly abnormal morphology, increased immune and fibroblast populations and YAP/TAZ-positive cells compared to control capsule. Capsule adjacent to radiated skin displayed abnormal collagen deposition, increased pro-inflammatory M1 macrophages, pro-fibrotic CD26+ fibroblasts, YAP/TAZ-positive cells and expression of IL-6, collagen type 1,TGF-β, YAP/TAZ in comparison to capsule that developed adjacent to radiated muscle. CONCLUSION: In this study, we show that the radiated tissue microenvironment significantly alters the inflammatory and fibrotic phenotype of human breast implant capsule. We also identify a novel link between radiation-induced capsular contracture and YAP/TAZ mechanical signaling which is further modified depending on the adjacent tissue type. This knowledge may help improve our understanding of the pathoaetiology of RICC and identifies a potential molecular therapeutic target to improve breast reconstructive outcomes in this high-risk population.© 2026. Plastic Surgery Research Council | All rights reserved |*Source: https://ps-rc.org/meeting/Program/2026/OS01.cgi*
title The Adjacent Tissue Environment And Mechanical Signaling Influence Radiation-induced Capsule Fibrosis
topic plastic surgery
reconstructive surgery
conference abstract
PSRC 2026
url https://doi.org/10.5281/zenodo.20061733