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2021
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| Online Access: | https://doi.org/10.5281/zenodo.5810197 |
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| _version_ | 1866901275170308096 |
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| author | Elena Moreno Giménez |
| author_facet | Elena Moreno Giménez |
| contents | <p>pISA-tree file path: _p_SUSPHIRE\_I_T12_CuInducible\_S_P1_TransientExp_Nb\_A_AgroinfFinalConstructsdCas9EV-GCMS\output\raw</p> <p>Title: Agroinfiltration of final constructs for copper-inducible pathway in <em>Nicotiana benthamiana</em> leaves<br> Description: Two constructs were made containing the three genes of the moth pheromone pathway under the regulation of synthetic promoters made in T11, nptII selection marker and with/without the gRNA1 for activation. In parallel, another construct containing the copper-inducible dCas9EV system with/without the guide was made in pLX vector so it could be co-transformed in the same agrobacterium colony with the moth pheromone pathway. Such Agrobacterium containing both plasmids (guided/nonguided pathway + nonguided/guided copper-inducible dCas9EV) was agroinfiltrated before stable transformation in <em>Nicotiana benthamiana</em> to check the expression of the genes.<br> </p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_5810197 |
| institution | Zenodo |
| language | |
| publishDate | 2021 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | _A_AgroinfFinalConstructsdCas9EV-GCMS.output.raw Elena Moreno Giménez SUSPHIRE <p>pISA-tree file path: _p_SUSPHIRE\_I_T12_CuInducible\_S_P1_TransientExp_Nb\_A_AgroinfFinalConstructsdCas9EV-GCMS\output\raw</p> <p>Title: Agroinfiltration of final constructs for copper-inducible pathway in <em>Nicotiana benthamiana</em> leaves<br> Description: Two constructs were made containing the three genes of the moth pheromone pathway under the regulation of synthetic promoters made in T11, nptII selection marker and with/without the gRNA1 for activation. In parallel, another construct containing the copper-inducible dCas9EV system with/without the guide was made in pLX vector so it could be co-transformed in the same agrobacterium colony with the moth pheromone pathway. Such Agrobacterium containing both plasmids (guided/nonguided pathway + nonguided/guided copper-inducible dCas9EV) was agroinfiltrated before stable transformation in <em>Nicotiana benthamiana</em> to check the expression of the genes.<br> </p> |
| title | _A_AgroinfFinalConstructsdCas9EV-GCMS.output.raw |
| topic | SUSPHIRE |
| url | https://doi.org/10.5281/zenodo.5810197 |