Aspergillus niger NRRL
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2021
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| _version_ | 1866902087850262528 |
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| author | Eliwa, Duaa Albadry, Mohamed A. Ibrahim, Abdel-Rahim S. Kabbash, Amal Meepagala, Kumudini Khan, Ikhlas A. El-Aasr, Mona Ross, Samir A. |
| author_facet | Eliwa, Duaa Albadry, Mohamed A. Ibrahim, Abdel-Rahim S. Kabbash, Amal Meepagala, Kumudini Khan, Ikhlas A. El-Aasr, Mona Ross, Samir A. |
| contents | <p><i>3.5. Microbial transformation using Aspergillus niger NRRL 322</i></p><p>Biotransformation of papaverine using <i>Aspergillus niger</i> NRRL 322 afforded to two metabolites (2 and 3). The residue (1 gm) was dissolved in 50 ml of methanol/dichloromethane mixture (1:1) and adsorbed onto 1 gm Celite and dried. The adsorbed sample was placed onto a glass column (50 × 2.5 cm) packed with silica after making a slurry in dichloromethane. The column was isocratically eluted with dichloromethane: methanol (97.5:2.5) and 10 ml fractions were collected. Fractions 20–44 were pooled together to give mixture of <b>2</b> and <b>3</b> as determined by TLC. The metabolites were obtained in the form of yellowish residue (80 mg, <i>R</i> f 0.3 S 2).</p><p>The mixture was loaded in silica biotage column (18 × 150 mm). The column was gradiently eluted with dichloromethane 100% then dichloromethane:2-propanol (98:2, 97:3,95:5) and 20 ml fractions were collected. Fractions 27–31 were pooled together to give pure <b>2</b>, in the form of white powder (7 mg, <i>Rf</i> 0.70 S 1). Fractions 65–85 were pooled together to give pure <b>3</b>, in the form of white powder (30 mg, <i>R</i> f 0.61 S 1).</p> |
| format | Recurso digital |
| id | zenodo_https___doi_org_10_5281_zenodo_8302180 |
| institution | Zenodo |
| language | |
| publishDate | 2021 |
| publisher | Zenodo |
| record_format | zenodo |
| spellingShingle | Aspergillus niger NRRL Eliwa, Duaa Albadry, Mohamed A. Ibrahim, Abdel-Rahim S. Kabbash, Amal Meepagala, Kumudini Khan, Ikhlas A. El-Aasr, Mona Ross, Samir A. Biodiversity Taxonomy Fungi Ascomycota Eurotiomycetes Eurotiales Aspergillaceae Aspergillus Aspergillus niger <p><i>3.5. Microbial transformation using Aspergillus niger NRRL 322</i></p><p>Biotransformation of papaverine using <i>Aspergillus niger</i> NRRL 322 afforded to two metabolites (2 and 3). The residue (1 gm) was dissolved in 50 ml of methanol/dichloromethane mixture (1:1) and adsorbed onto 1 gm Celite and dried. The adsorbed sample was placed onto a glass column (50 × 2.5 cm) packed with silica after making a slurry in dichloromethane. The column was isocratically eluted with dichloromethane: methanol (97.5:2.5) and 10 ml fractions were collected. Fractions 20–44 were pooled together to give mixture of <b>2</b> and <b>3</b> as determined by TLC. The metabolites were obtained in the form of yellowish residue (80 mg, <i>R</i> f 0.3 S 2).</p><p>The mixture was loaded in silica biotage column (18 × 150 mm). The column was gradiently eluted with dichloromethane 100% then dichloromethane:2-propanol (98:2, 97:3,95:5) and 20 ml fractions were collected. Fractions 27–31 were pooled together to give pure <b>2</b>, in the form of white powder (7 mg, <i>Rf</i> 0.70 S 1). Fractions 65–85 were pooled together to give pure <b>3</b>, in the form of white powder (30 mg, <i>R</i> f 0.61 S 1).</p> |
| title | Aspergillus niger NRRL |
| topic | Biodiversity Taxonomy Fungi Ascomycota Eurotiomycetes Eurotiales Aspergillaceae Aspergillus Aspergillus niger |
| url | https://doi.org/10.5281/zenodo.8302180 |