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author Miao, Lei
Lou, Jinfang
Xu, Sicong
Zhang, Jinzhu
Zhong, Yongqing
Wang, Guojian
Li, Yuanyuan
Lei, Shaowei
Shao, Shuai
Wang, Jianghai
Huang, Yun
Tang, Xinyi
Ding, Wanjing
Ma, Zhongjun
author_facet Miao, Lei
Lou, Jinfang
Xu, Sicong
Zhang, Jinzhu
Zhong, Yongqing
Wang, Guojian
Li, Yuanyuan
Lei, Shaowei
Shao, Shuai
Wang, Jianghai
Huang, Yun
Tang, Xinyi
Ding, Wanjing
Ma, Zhongjun
Miao, Lei
Lou, Jinfang
Xu, Sicong
Zhang, Jinzhu
Zhong, Yongqing
Wang, Guojian
Li, Yuanyuan
Lei, Shaowei
Shao, Shuai
Wang, Jianghai
Huang, Yun
Tang, Xinyi
Ding, Wanjing
Ma, Zhongjun
collection PubMed - marine biology
contents Discovery of New Difluorocyclobutyl Derivatives as Effective Glucagon-Like Peptide-1 Receptor Agonists with Reduced hERG Inhibitory Activities. Miao, Lei Lou, Jinfang Xu, Sicong Zhang, Jinzhu Zhong, Yongqing Wang, Guojian Li, Yuanyuan Lei, Shaowei Shao, Shuai Wang, Jianghai Huang, Yun Tang, Xinyi Ding, Wanjing Ma, Zhongjun Animals Humans Male Mice Blood Glucose Cricetulus Drug Discovery ERG1 Potassium Channel Ether-A-Go-Go Potassium Channels Glucagon-Like Peptide-1 Receptor Glucagon-Like Peptide-1 Receptor Agonists HEK293 Cells Hypoglycemic Agents Piperidines Structure-Activity Relationship Benzimidazoles Danuglipron (PF-06882961), a small-molecule agonist of the glucagon-like peptide-1 receptor (GLP-1R) developed by Pfizer, has shown significant potential to reduce blood glucose levels and weight in patients with type 2 diabetes mellitus. However, it has moderate hERG inhibitory activities (IC = 4.3 μM), potentially conferring a risk for cardiac toxicity. Here, we report a new class of difluorocyclobutyl derivatives that can be used to reduce the potential hERG inhibition caused by the piperidine ring of danuglipron. After in vitro and in vivo screening, compound was found to be the most potent GLP-1R agonist, with an EC of 0.048 nM. Furthermore, compound showed preferable absorption and excellent β-arrestin pathway selectivity compared with danuglipron. In the glucose tolerance test, compound effectively inhibited elevated blood glucose levels. These results indicate that compound is a promising GLP-1R agonist.
format Artículo científico
id pubmed_40167442
institution PubMed
language en
publishDate 2025
publisher Journal of medicinal chemistry
record_format pubmed
spellingShingle Discovery of New Difluorocyclobutyl Derivatives as Effective Glucagon-Like Peptide-1 Receptor Agonists with Reduced hERG Inhibitory Activities.
Miao, Lei
Lou, Jinfang
Xu, Sicong
Zhang, Jinzhu
Zhong, Yongqing
Wang, Guojian
Li, Yuanyuan
Lei, Shaowei
Shao, Shuai
Wang, Jianghai
Huang, Yun
Tang, Xinyi
Ding, Wanjing
Ma, Zhongjun
Animals
Humans
Male
Mice
Blood Glucose
Cricetulus
Drug Discovery
ERG1 Potassium Channel
Ether-A-Go-Go Potassium Channels
Glucagon-Like Peptide-1 Receptor
Glucagon-Like Peptide-1 Receptor Agonists
HEK293 Cells
Hypoglycemic Agents
Piperidines
Structure-Activity Relationship
Benzimidazoles
Discovery of New Difluorocyclobutyl Derivatives as Effective Glucagon-Like Peptide-1 Receptor Agonists with Reduced hERG Inhibitory Activities. Miao, Lei Lou, Jinfang Xu, Sicong Zhang, Jinzhu Zhong, Yongqing Wang, Guojian Li, Yuanyuan Lei, Shaowei Shao, Shuai Wang, Jianghai Huang, Yun Tang, Xinyi Ding, Wanjing Ma, Zhongjun Animals Humans Male Mice Blood Glucose Cricetulus Drug Discovery ERG1 Potassium Channel Ether-A-Go-Go Potassium Channels Glucagon-Like Peptide-1 Receptor Glucagon-Like Peptide-1 Receptor Agonists HEK293 Cells Hypoglycemic Agents Piperidines Structure-Activity Relationship Benzimidazoles Danuglipron (PF-06882961), a small-molecule agonist of the glucagon-like peptide-1 receptor (GLP-1R) developed by Pfizer, has shown significant potential to reduce blood glucose levels and weight in patients with type 2 diabetes mellitus. However, it has moderate hERG inhibitory activities (IC = 4.3 μM), potentially conferring a risk for cardiac toxicity. Here, we report a new class of difluorocyclobutyl derivatives that can be used to reduce the potential hERG inhibition caused by the piperidine ring of danuglipron. After in vitro and in vivo screening, compound was found to be the most potent GLP-1R agonist, with an EC of 0.048 nM. Furthermore, compound showed preferable absorption and excellent β-arrestin pathway selectivity compared with danuglipron. In the glucose tolerance test, compound effectively inhibited elevated blood glucose levels. These results indicate that compound is a promising GLP-1R agonist.
title Discovery of New Difluorocyclobutyl Derivatives as Effective Glucagon-Like Peptide-1 Receptor Agonists with Reduced hERG Inhibitory Activities.
topic Animals
Humans
Male
Mice
Blood Glucose
Cricetulus
Drug Discovery
ERG1 Potassium Channel
Ether-A-Go-Go Potassium Channels
Glucagon-Like Peptide-1 Receptor
Glucagon-Like Peptide-1 Receptor Agonists
HEK293 Cells
Hypoglycemic Agents
Piperidines
Structure-Activity Relationship
Benzimidazoles
url https://pubmed.ncbi.nlm.nih.gov/40167442/